Abstract
A cold-regulated gene cluster, rbpA-rpsU, was isolated by differential screening in the cyanobacterium Anabaena variabilis M3. The two genes were co-transcribed as a 900 nt transcript. The level of this transcript was 5–10 times higher in cells grown at 22°C than in those grown at 38°C. The RbpA protein, which was homologous to plant glycine-rich proteins, exhibited a strong affinity to RNA from this cyanobacterium.
This is a preview of subscription content, log in via an institution to check access.
References
Bar-Zvi D, Shagan T, Schindler U, Cashmore AR: RNP-T, a ribonucleoprotein from Arabidopsis thaliana, contains two RNP-80 motifs and a novel acidic repeat arranged in an α-helix conformation. Plant Mol Biol 20: 833–838 (1992).
Gao GJ, Sarkar N: Poly(A) RNA in Escherichia coli: nucleotide sequence at the junction of the lpp transcript and the polyadenylate moiety. Proc Natl Acad Sci USA 89: 7456–7550 (1992).
DeLisle AJ: RNA-binding protein from Arabidopsis. Plant Physiol 102: 313–314 (1993).
Didierjean L, Frendo P, Burkard G: Stress responses in maize: Sequence analysis of cDNAs encoding glycine-rich proteins. Plant Mol Biol 18: 847–849 (1992).
Goldstein J, Pollitt NS, Inouye M: Major cold shock protein of Escherichia coli. Proc Natl Acad Sci USA 87: 283–287 (1990).
Gomez J, Sanchez-Martinez D, Stiefel V, Rigau J, Puigdomenech P, Pages M: A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334: 262–264 (1988).
Ingraham J: Effect of temperature, pH, water activity, and pressure on growth. In: Neidhardt F (ed) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, pp. 1543–1554. American Society for Microbiology, Washington, D. C. (1987).
Jones PG, Krah R, Tafuri SR, Wolffe AP: DNA gyrase, CS7.4, and the cold shock response in Escherichia coli. J Bact 174: 5798–5802 (1992).
Jones PG, VanBogelen RA, Neidhardt FC: Induction of proteins in response to low temperature in Escherichia coli. J Bact 169: 2092–2095 (1987).
Kenan DJ, Query CC, Keene JD: RNA recognition: towards identifying determinants of specificity. Trends Biochem Sci 16: 214–220 (1991).
Lupski JR, Smiley BL, Godson GN: Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12. Mol Gen Genet 189: 48–57 (1983).
Neidhardt FC, VanBogelen RA: Heat shock response. In: Neidhardt F (ed) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, pp. 1334–1345. American Society for Microbiology, Washington, D. C. (1987).
Sato N: Cloning of a low-temperature-induced gene lti2 from the cyanobacterium Anabaena variabilis M3 that is homologous to α-amylases. Plant Mol Biol 18: 165–170 (1992).
Sato N, Murata N: Lipid biosynthesis in the blue-green alga, Anabaena variabilis. II. Fatty acids and lipid molecular species. Biochim Biophys Acta 710: 279–289 (1982).
Sato N, Seyama Y, Murata N: Lipid-linked desaturation of palmitic acid in monogalactosyl diacylglycerol in the blue-green alga (cyanobacterium) Anabaena variabilis studied in vivo. Plant Cell Physiol 27: 819–835 (1986).
Schindelin H, Marahiel MA, Heinemann U: Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein. Nature 364: 164–168 (1993).
Schnuchel A, Wiltscheck R, Czisch M, Herrier M, Willimsky G, Graumann P, Marahiel MA, Holak TA: Structure in solution of the major cold-shock protein from Bacillus subtilis. Nature 364: 169–171 (1993).
Sturm A: A wound-inducible glycine-rich protein from Daucus carota with homology to single-stranded nucleic acid-binding proteins. Plant Physiol 99: 1689–1692 (1992).
Sugita M, Sugiura M: Nucleic acid-binding proteins containing a consensus sequence-type RNA-binding domain of the cyanobacterium Anacystis nidulans. In: Murata N (ed) Research in Photosynthesis, Vol. 3, pp. 279–282. Kluwer Academic Publishers, Dordrecht (1992).
Vierling E: The roles of heat shock proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 42: 579–620 (1991).
Wagner AF, Bugianesi RL, Shen TY: Preparation of Sepharose-bound poly(rI:rC). Biochem Biophys Res Commun 45: 184–189 (1971).
Willimsky G, Bang H, Fischer G, Marahiel MA: Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. J Bacteriol 174: 6326–6335 (1992).
Ye L, Li Y, Fukami-Kobayashi K, Go M, Konishi T, Watanabe A, Sugiura M: Diversity of a ribonucleoprotein family in tobacco chloroplasts: two new chloroplast ribonucleoproteins and a phylogenetic tree of ten chloroplast RNA-binding domains. Nucl Acids Res 19: 6485–6490 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sato, N. A cold-regulated cyanobacterial gene cluster encodes RNA-binding protein and ribosomal protein S21. Plant Mol Biol 24, 819–823 (1994). https://doi.org/10.1007/BF00029864
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029864