4. Conclusion
The data presented, demonstrate for the first time PEP turnover of HN by a limited postcysteine as well as the expected post-proline proteolysis demonstrated in cell extract resulting in the inactivation of this potentially apoptosis-related factor. These findings lead to the hypothesis of a PEP-mediated control of HN homeostasis maintaining neuronal cell survival. This implicates a novel use of PEP inhibitors potentially preventing intracellular HN digestion. Consequently, PEP-inhibition might be a new target for apoptosis prevention.
This study further uncovered a so far unknown enzymatic specificity for a post-cysteine cleavage of the mammalian exopeptidases DP2, DP4, DP8 and DP9 and the endopeptidase PEP.
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Bär, J.W. et al. (2006). Prolyl Endopeptidase Cleaves the Apoptosis Rescue Peptide Humanin and Exhibits an Unknown Post-Cysteine Cleavage Specificity. In: Lendeckel, U., Reinhold, D., Bank, U. (eds) Dipeptidyl Aminopeptidases. Advances in Experimental Medicine and Biology, vol 575. Springer, Boston, MA . https://doi.org/10.1007/0-387-32824-6_11
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