Abstract
The Respiration Quotient (RQ) is a key metabolic parameter for cell cultures and is usually determined from gas analysis only. In bicarbonate buffered media the carbon dioxide balance is affected by accumulation and hence the RQ can not directly be calculated from gas measurements. A Kalman Filter as software sensor that estimates the CER can cope with these buffering capacities and thus is used for determining the RQ. The model used by the Kalman Filter lumps all carbonate in the liquid to one term in order to eliminate the role of a priori knowledge of cell and medium kinetics without affecting the performance.
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© 2001 Kluwer Academic Publishers
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Neeleman, R. (2001). Respiration Quotient: Estimation During Batch Cultivation in Bicarbonate Buffered Media. In: Hofman, M., Thonart, P. (eds) Engineering and Manufacturing for Biotechnology. Focus on Biotechnology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/0-306-46889-1_13
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DOI: https://doi.org/10.1007/0-306-46889-1_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6927-1
Online ISBN: 978-0-306-46889-6
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