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Diagnostics for Citrus Greening Disease (Huanglongbing): Current and Emerging Technologies

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Plant Biotechnology: Progress in Genomic Era

Abstract

Huanglongbing (HLB) or ‘citrus greening’ is the most destructive disease in citrus production worldwide and no cure is available at present. This disease is caused by a phloem-limited, non-cultured, Gram-negative alpha-proteobacteria, Candidatus Liberibacter spp., vectored by the psyllids, Diaphorina citri and Trioza erytreae. Practically all commercial citrus species and cultivars are sensitive, regardless of rootstocks used. Foliar symptoms produced by HLB can easily go unnoticed and often be confused with those induced by nutrient deficiencies or other endemic diseases. Low concentration and the uneven spatial and temporal distribution of the pathogen in host plants and insect vectors make the detection even more difficult. Currently molecular techniques based on polymerase chain reaction are mainly used for the identification of HLB disease, which requires exhaustive sampling and processing operations. Furthermore, researches are ongoing in spectroscopic and imaging techniques, profiling of plant volatile organic compounds, and isothermal amplification approaches. This review provides a comprehensive overview on the various diagnostic techniques available for HLB and also discusses the recent advancements in HLB disease detection.

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Authors and Affiliations

  1. Plant Pathology Lab, ICAR-Central Citrus Research Institute, Nagpur, Maharashtra, India

    Ashis K. Das

  2. ICAR Research Complex for NEH Region, Manipur Centre, Imphal, Manipur, India

    Susheel K. Sharma

  3. School of Plant Sciences, University of Arizona, Tucson, AZ, USA

    Neha Thakre

Authors
  1. Ashis K. Das
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  2. Susheel K. Sharma
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  3. Neha Thakre
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Correspondence to Ashis K. Das .

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Editors and Affiliations

  1. Amity Institute of Biotechnology, Amity University, Gurgaon, Haryana, India

    S. M. Paul Khurana

  2. Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India

    Rajarshi Kumar Gaur

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Das, A.K., Sharma, S.K., Thakre, N. (2019). Diagnostics for Citrus Greening Disease (Huanglongbing): Current and Emerging Technologies. In: Khurana, S., Gaur, R. (eds) Plant Biotechnology: Progress in Genomic Era. Springer, Singapore. https://doi.org/10.1007/978-981-13-8499-8_26

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