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Expression of renin-angiotensin system and extracellular matrix genes in cardiovascular cells and its regulation through AT1 receptor

  • Chapter
Control of Gene Expression by Catecholamines and the Renin-Angiotensin System

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 33))

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Abstract

Angiotensinogen (AGT) is a unique substrate of the renin-angiotensin system and fibronectin (FN) is an important component of the extracellular matrix. These play critical roles in the pathophysiological changes including cardiovascular remodeling and hypertrophy in response to hypertension. This study was performed to examine the regulation of AGT and FN gene in cardiac myocytes (CMs) and vascular smooth muscle cells (VSMCs) in response to mechanical stretch. Mechanical stretch significantly increased the AGT mRNA expression in CMs, while these stimuli did not affect FN mRNA levels. On the other hand, mechanical stretch upregulated FN mRNA levels in VSMCs, whereas no increase in AGT mRNA levels was observed in response to stretch stimuli. An angiotensin II type 1 (AT1) receptor antagonist (CV 11974) significantly decreased these stretch-mediated increases in mRNA level and promoter activity of the AGT and FN gene, whereas angiotensin II type 2 (AT2) receptor antagonist (PD123319) did not affect the induction. These results indicate that mechanical stretch activates transcription of the AGT and FN gene mainly via AT1 receptor-pathway in CMs and VSMCs. Furthermore, mechanisms regulating AGT and FN gene seem to be different between CMs and VSMCs. (Mol Cell Biochem 212: 203–209, 2000)

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Authors and Affiliations

  1. Department of Internal Medicine II, Japan

    Kouichi Tamura, Nobuo Nyui, Izumi Takasaki & Satoshi Umemura

  2. Cardiovascular Research, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, USA

    Kouichi Tamura, Yuqing E. Chen, Qin Chen, Masatsugu Horiuchi, Richard E. Pratt & Victor J. Dzau

  3. Department of Dermatology, Yokohama City University School of Medicine, Yokohama, Japan

    Nobuko Tamura

  4. Department of Internal Medicine II, Yokahama City University School of Medicine, 3-9, Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan

    Kouichi Tamura

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  1. Kouichi Tamura
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Editors and Affiliations

  1. Internal Medicine and Cardiology, Molecular Cardiology Laboratory, Philipps University of Marburg, Karl-von-Frisch-Strasse 1, 35044, Marburg, Germany

    Heinz Rupp

  2. Internal Medical, Molecular Cardiology Laboratory, Philipps University of Marburg, Karl-von-Frisch-Strasse, 35033, Marburg, Germany

    Bernard Maisch

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Tamura, K. et al. (2000). Expression of renin-angiotensin system and extracellular matrix genes in cardiovascular cells and its regulation through AT1 receptor. In: Rupp, H., Maisch, B. (eds) Control of Gene Expression by Catecholamines and the Renin-Angiotensin System. Developments in Molecular and Cellular Biochemistry, vol 33. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4351-0_23

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  • DOI: https://doi.org/10.1007/978-1-4615-4351-0_23

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6955-4

  • Online ISBN: 978-1-4615-4351-0

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