Abstract
We have previously demonstrated directly, by chromatographic separation, the coexistence of multiple immunoglobulin activities against the TSH-receptor (Gig) in sera from patients with Graves’ disease (Worthington et al. 1986). Biological properties of these Gig varied from blocking to stimulating with the mix present in an individual being unpredictable. These components were all detected initially using a radioreceptor assay which employs the inhibition of -TSH from binding to solubilised receptors. We have therefore, examined the interaction of mixtures of 125I-TSH and the purified Gig with the TSH-receptor in order to clarify the relationship between structure and function for these antibodies.
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References
Southgate K., Creagh F.M., Teec, M, Kingswood C. and Rees-Smith B., 1984, A receptor assay for the measurement of TSH-receptor antibodies in unextracted serum. Clin Endocrinol 20:539–543.
Worthington J., Himsworth R.L. and Byfield P.G.H., 1986, Isolation of both agonist and nonagonist Graves’ immunoglobulins from the sera of individual patients, in: “The Thyroid and Autoimmunity”, H.A. Drexhage and W.M. Wiersinga, eds., Elsevier, Amsterdam.
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© 1987 Plenum Press, New York
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Byfield, P.G.H., Worthington, J. (1987). Interaction of Purified Graves’ Immunoglobulins with the TSH-Receptor. In: Pinchera, A., Ingbar, S.H., McKenzie, J.M., Fenzi, G.F. (eds) Thyroid Autoimmunity. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0945-1_51
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DOI: https://doi.org/10.1007/978-1-4613-0945-1_51
Publisher Name: Springer, Boston, MA
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