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Transient EPR Spectroscopy as Applied to Light-Induced Functional Intermediates Along the Electron Transfer Pathway in Photosystem I

  • Chapter
Photosystem I

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 24))

Abstract

In the pursuit to understand biological function in fundamental processes such as photo-induced charge separation along a chain of electron carriers as in Photosystem I (PS I), detailed knowledge of protein-cofactor interactions is desired in real time. Today, every promising time resolved molecular spectroscopic method is scrutinized, adapted, and exploited to provide that knowledge. The primary processes in photosynthetic charge separation involve the sequential generation of paramagnetic intermediates in the form of radical ion pairs. Hence, magnetic resonance techniques are a specific method of choice and promise the most detailed insight into protein-induced molecular interactions.

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References

  • Amesz J (1995) The heliobacteria, a new group of photosynthetic bacteria. J Photochem Photobiol B 30: 89–96

    Article  CAS  Google Scholar 

  • Angerhofer A and Bittl R (1996) Radicals and radical pairs in photosynthesis. Photochem Photobiol 63: 11–38

    CAS  Google Scholar 

  • Angstl R (1990) Calculation of molecular g tensors. Comparison of Rayleigh–Schroedinger and Hartree–Fock perturbation theory. Chem Phys 145: 413–426

    Article  CAS  Google Scholar 

  • Bell J (1993) Bertlmann′ socks and the nature of reality. In: Bell J (ed) Speakable and Unspeakable in Quantum Mechanics, pp 139–158. Cambridge University Press, Cambridge

    Google Scholar 

  • Biggins J and Mathis P (1988) Functional role of vitamin K in photosystem I of the cyanobacterium Synechocystis 6803. Biochemistry 27: 1494–1500

    Article  PubMed  CAS  Google Scholar 

  • Bittl R and Kothe G (1991) Transient EPR of radical pairs in photosynthetic reaction centers –prediction of quantum beats. Chem Phys Lett 177: 547–553

    Article  CAS  Google Scholar 

  • Bittl R and Weber S (2005) Transient radical pairs studied by time-resolved EPR. Biochim Biophys Acta 1707: 117–126

    Article  PubMed  CAS  Google Scholar 

  • Bittl R and Zech SG (1997) Pulsed EPR study of spin-coupled radical pairs in photosynthetic reaction centers: measurement of the distance between P700 + and A1 - in photosystem I and between P865 + and QA - in bacterial reaction centers. J Phys Chem B 101: 1429–1436

    Article  CAS  Google Scholar 

  • Bittl R and Zech SG (2001) Pulsed EPR spectroscopy on short-lived intermediates in Photosystem I. Biochim Biophys Acta 1507: 194–211

    Article  PubMed  CAS  Google Scholar 

  • Bittl R, van der Est A, Kamlowski A, Lubitz W and Stehlik D (1994) Time-resolved EPR of the radical pair P865 +QA - in bacterial reaction centers. Observation of transient mutations, quantum beats and envelope modulation effects. Chem Phys Lett 226: 349–358

    Article  CAS  Google Scholar 

  • Blankenship R (1992) Origin and early evolution of photosynthesis. Photosynth Res 33: 91–111

    Article  PubMed  CAS  Google Scholar 

  • Blankenship R, McGuire A and Sauer K (1975) Chemically induced dynamic electron polarization in chloroplasts at room temperature: evidence for triplet state participation in photosynthesis. Proc Natl Acad Sci USA 72: 4943–4947

    Article  PubMed  CAS  Google Scholar 

  • Bock CH, van der Est AJ, Brettel K and Stehlik D (1989) Nanosecond electron transfer kinetics in photosystem I as obtained from transient EPR at room temperature. FEBS Lett 247: 91–96

    Article  CAS  Google Scholar 

  • Bohm D (1951) Quantum Theory, pp. 611–623. Englewood-Cliff, New York

    Google Scholar 

  • Bratt PJ, Rohrer M, Krzystek J, Evans MCW, Brunel LC and Angerhofer A (1997) Submillimeter high-field EPR studies of the primary donor in plant photosystem I P700. J Phys Chem B 101: 9686–9689

    Article  CAS  Google Scholar 

  • Brettel K (1988) Electron transfer from A1 - to an iron–sulfur center with t1 / 2 = 200 ns at room temperature in Photosystem I. FEBS Lett 239: 93–98

    Article  CAS  Google Scholar 

  • Brettel K (1997) Electron transfer and arrangement of the redox cofactors in photosystem I. Biochim Biophys Acta 1318: 322–373

    Article  CAS  Google Scholar 

  • Brettel K and Leibl W (2001) Electron transfer in Photosystem I. Biochim Biophys Acta 1507: 100–114

    Article  PubMed  CAS  Google Scholar 

  • Buckley CD, Hunter DA, Hore PJ and McLauchlan KA (1987) Electron spin resonance of spin-correlated radical pairs. Chem Phys Lett 135: 307–312

    Article  CAS  Google Scholar 

  • Budil DE and Thurnauer MC (1991) The chlorophyll triplet state as a probe of structure and function in photosynthesis. Biochim Biophys Acta 1057: 1–41

    Article  PubMed  CAS  Google Scholar 

  • Closs GL, Forbes MDE and Norris JR (1987) Spin-polarized electron paramagnetic resonance spectra of radical pairs in micelles. Observation of electron spin–spin interactions. J Phys Chem 91: 3592–3599

    Article  CAS  Google Scholar 

  • Deligiannakis Y and Rutherford AW (2001) Electron spin echo envelope modulation spectroscopy in photosystem I. Biochim Biophys Acta 1507: 226–246

    Article  PubMed  CAS  Google Scholar 

  • Dutton P, Leigh JS and Seibert M (1972) Primary processes in photosynthesis. In situ ESR studies on the light-induced oxidized and triplet state of reaction center bacteriochlorophyll. Biochem Biophys Res Commun 46: 406–413

    Article  PubMed  CAS  Google Scholar 

  • Evans MCW and Nugent JHA (1993) Structure and function of the reaction center cofactors in oxygenic organisms. In: Deisenhofer J and Norris J (eds) The Photosynthetic Reaction Center. Academic Press, San Diego

    Google Scholar 

  • Ferreira KN, Iverson TM, Maghlaoui K, Barber J and Iwata S (2004) Architecture of the photosynthetic oxygen-evolving center. Science 303: 1831–1838

    Article  PubMed  CAS  Google Scholar 

  • Furrer R and Thurnauer MC (1983) Resolution of signals attributed to Photosystem-I primary reactants by time-resolved electron-paramagnetic-resonance at K-band. FEBS Lett 153: 399–403

    Article  CAS  Google Scholar 

  • Golbeck JH (1987) Structure, function and organization of the Photosystem I reaction center complex. Biochim Biophys Acta 895: 167–204

    PubMed  CAS  Google Scholar 

  • Grimaldi S, Ostermann T, Weiden N, Mogi T, Miyoshi H, Ludwig B, Michel H, Prisner T and MacMillan F (2003) Asymmetric binding of the high-affinity QH- ubisemiquinone in quinol oxidase (bo3) from Escherichia coli studied by multifrequency electron paramagnetic resonance spectroscopy. Biochemistry 42: 5632–5639

    Article  PubMed  CAS  Google Scholar 

  • Grinberg O and Berliner L (2004) Very High Frequency (VHF) ESR/EPR. Kluwer/Plenum Publishers, New York

    Google Scholar 

  • Guergova-Kuras M, Boudreaux B, Joliot A, Joliot P and Redding K (2001) Evidence for two active branches for electron transfer in Photosystem I. Proc Natl Acad Sci USA 98: 4437–4442

    Article  PubMed  CAS  Google Scholar 

  • Haffa ALM, Lin S, Williams JC, Bowen BP, Taguchi AKW, Allen JP and Woodbury NW (2004) Controlling the pathway of photosynthetic charge separation in bacterial reaction centers. J Phys Chem 108: 4–7

    CAS  Google Scholar 

  • Hanley J, Deligiannakis Y, MacMillan F, Bottin H and Rutherford AW (1997) ESEEM study of the phyllosemiquinone radical A1 - in 14N- and 15N-labeled Photosystem I. Biochemistry 36: 11543–11549

    Article  PubMed  CAS  Google Scholar 

  • Heinen U, Golecki JR, Poluektov OG, Berthold T, Schlesselman SL, Frezzato D, Ohmes E, Moro GJ, Thurnauer M and Kothe G (2004a) Magnetic field induced orientation of photosynthetic reaction centers as revealed by time-resolved W-band EPR of spin-correlated radical pairs. III. Development of a molecular model. Appl Magn Reson 26: 99–115

    CAS  Google Scholar 

  • Heinen U, Poluektov OG, Stavitski E, Berthold T, Ohmes E, Schlesselman SL, Golecki JR, Moro GJ, Levanon H, Thurnauer M and Kothe G (2004b) Magnetic field induced orientation of photosynthetic reaction centers as revealed by time-resolved D-band EPR of spin-correlated radical pairs. II. Field dependence of the alignment. J Phys Chem B 108: 9498–9504

    Article  CAS  Google Scholar 

  • Heinnickel M, Shen G, Agalarov R and Golbeck JH (2005) Resolution and reconstitution of a bound Fe–S protein from the photosynthetic reaction center of Heliobacterium modesticaldum. Biochemistry 44: 9950–9960

    Article  PubMed  CAS  Google Scholar 

  • Hoff A (1984) Electron spin polarization of photosynthetic reactants. Q Rev Biophys 17: 153–282

    Article  PubMed  CAS  Google Scholar 

  • Hoff A and Deisenhofer J (1997) Photophysics of photosynthesis. Structure and spectroscopy of reaction centers of purple bacteria. Phys Rep 287: 1–247

    Article  CAS  Google Scholar 

  • Hore PJ (1989) Analysis of polarized EPR spectra. In: Hoff AJ (ed) Advanced EPR in Biology and Biochemistry, Chapter 12, pp 405–440. Elsevier, Amsterdam

    Google Scholar 

  • Huber M, Törring J, Plato M, Fink U, Lubitz W, Feick R, Schenck C and Möbius K (1995) Investigation of the electronic structure of the primary electron donor in bacterial photosynthesis –measurements of the anisotropy of the electronic g-tensor using high-field/high-frequency EPR. Sol Energy Mater Sol Cells 38: 119–126

    Article  CAS  Google Scholar 

  • Ishikita H and Knapp EW (2003) Redox potential of quinones in both electron transfer branches of photosystem I. J Biol Chem 278: 52002–52011

    Article  PubMed  CAS  Google Scholar 

  • Itoh S, Iwaki M and Ikegami I (2001) Modification of Photosystem I reaction center by the extraction and exchange of chlorophylls and quinones. Biochim Biophys Acta 1507: 115–138

    Article  PubMed  CAS  Google Scholar 

  • Iwaki M and Itoh S (1991) Structure of the phylloquinone-binding QPh site in green plant photosystem I reaction centers: the affinity of quinones and quinoid compounds for the QPh site. Biochemistry 30: 5347–5352

    Article  PubMed  CAS  Google Scholar 

  • Jeschke G, Rakhmatullin R and Schweiger A (1998) Sensitivity enhancement by matched microwave pulses in one- and two-dimensional electron spin echo envelope modulation spectroscopy. J Magn Reson 131: 261–271

    Article  PubMed  CAS  Google Scholar 

  • Johnson TW, Zybailov B, Jones AD, Bittl R, Zech S, Stehlik D, Golbeck JH and Chitnis PR (2001) Recruitment of a foreign quinone into the A site of Photosystem I –in vivo replacement of plastoquinone-9 by media-supplemented naphthoquinones in phylloquinone biosynthetic pathway mutants of Synechocystis sp PCC 6803. J Biol Chem 276: 39512–39521

    Article  PubMed  CAS  Google Scholar 

  • Joliot P and Joliot A (1999) In vivo analysis of the electron transfer within Photosystem I: are the two phylloquinones involved? Biochemistry 38: 11130–11136

    Article  PubMed  CAS  Google Scholar 

  • Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauß N (2001) Three dimensional structure of Photosystem I at 2.5 Å resolution. Nature 411: 909–917

    Article  PubMed  CAS  Google Scholar 

  • Kamiya N and Shen JR (2003) Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7 Å resolution. Proc Natl Acad Sci USA 100: 98–103

    Article  PubMed  CAS  Google Scholar 

  • Kamlowski A, van der Est A, Fromme P, Krauß N, Schubert WD, Klukas O and Stehlik D (1997a) The structural organization of the PsaC protein in Photosystem I from single crystal EPR and x-ray crystallographic studies. Biochim Biophys Acta 1319: 199–213

    Article  CAS  Google Scholar 

  • Kamlowski A, van der Est A, Fromme P and Stehlik D (1997b) Low temperature EPR on Photosystem I single crystals: orientation of the iron–sulfur centers FA and FB. Biochim Biophys Acta 1319: 185–198

    Article  CAS  Google Scholar 

  • Kamlowski A, Altenberg-Greulich B, van der Est A, Zech SG, Bittl R, Fromme P, Lubitz W and Stehlik D (1998) The quinone acceptor A in photosystem I: binding site, and comparison to QA in purple bacteria reaction centers. J Phys Chem B 102: 8278–8287

    Article  CAS  Google Scholar 

  • Kandrashkin YE, Vollmann W, Stehlik D, Salikhov K and van der Est A (2002) The magnetic field dependence of the electron spin polarization in consecutive spin correlated radical pairs in type I photosynthetic reaction centres. Mol Phys 100: 1431–1443

    Article  CAS  Google Scholar 

  • Kirmaier C, Laible PD, Hanson DK and Holten D (2003) B-side charge separation in bacterial photosynthetic reaction centers: nanosecond time scale electron transfer from HB - to QB. Biochemistry 42: 2016–2024

    Article  PubMed  CAS  Google Scholar 

  • Kleinfeld D, Okamura MY and Feher G (1984) Electron-transfer kinetics in photosynthetic reaction centers cooled to cryogenic temperatures in the charge-separated state: evidence for light-induced structural changes. Biochemistry 23: 5780–5786

    Article  PubMed  CAS  Google Scholar 

  • Klette R, Törring J, Plato M, Möbius K, Boenigk B and Lubitz W (1993) Determination of the g tensor of the primary donor cation radical in single crystals of Rhodobacter sphaeroides R-26 reaction centers by 3-mm high-field EPR. J Phys Chem 97: 2015–2020

    Article  CAS  Google Scholar 

  • Kothe G, Weber S, Bittl R, Ohmes E, Thurnauer M and Norris J (1991) Transient EPR of light-induced radical pairs in plant photosystem: I. Observation of quantum beats. Chem Phys Lett 186: 474–480

    Article  CAS  Google Scholar 

  • Kothe G, Weber S, Ohmes E, Thurnauer MC and Norris JR (1994) High time resolution electron paramagnetic resonance of light-induced radical pairs in photosynthetic bacterial reaction centers: observation of quantum beats. J Am Chem Soc 116: 7729–7734

    Article  CAS  Google Scholar 

  • Krabben L, Schlodder E, Jordan R, Carbonera D, Giacometti G, Lee H, Webber AN and Lubitz W (2000) Influence of the axial ligands on the spectral properties of P700 of Photosystem I: a study of site-directed mutants. Biochemistry 39: 13012–13025

    Article  PubMed  CAS  Google Scholar 

  • Link G, Berthold T, Bechtold M, Weidner JU, Ohmes E, Tang J, Poluektov O, Utschig L, Schlesselman SL, Thurnauer MC and Kothe G (2001) Structure of the P700 +A1 - radical pair intermediate in photosystem I by high time resolution multifrequency electron paramagnetic resonance: analysis of quantum beat oscillations. J Am Chem Soc 123: 4211–4222

    Article  PubMed  CAS  Google Scholar 

  • Lubitz W and Feher G (1999) The primary and secondary acceptors in bacterial photosynthesis III. Characterization of the quinone radicals QA - and QB - by EPR and ENDOR. Appl Magn Reson 17: 1–48

    CAS  Google Scholar 

  • MacMillan F, Hanley J, vanderWeerd L, Knupling M, Un S and Rutherford AW (1997) Orientation of the phylloquinone electron acceptor anion radical in photosystem I. Biochemistry 36: 9297–9303

    Article  PubMed  CAS  Google Scholar 

  • Möbius K (2003) Multifrequency EPR and ENDOR on transient radicals and radical pairs in liquid crystals and proteins: structure and dynamics of cofactors in natural and artificial photosynthesis. Mol Cryst Liq Cryst 394: 1–17

    Article  CAS  Google Scholar 

  • Möbius K, Savitsky A and Fuchs M (2004) Primary processes in photosynthesis: what do we learn from high-field EPR spectroscopy? In: Grinberg O and Berliner L (eds) Very High Frequency (VHF) ESR/EPRL, pp 45–93. Kluwer/Plenum Publisher, New York

    Google Scholar 

  • Nitschke W and Rutherford AW (1991) Photosynthetic reaction centres: variations on a common structural theme? Trends Biochem Sci 16: 241–245

    Article  PubMed  CAS  Google Scholar 

  • Paddock ML, Chang C, Xu Q, Abresch EC, Axelrod HL, Feher G and Okamura MY (2005) Quinone (QB) reduction by B-branch electron transfer in mutant bacterial reaction centers from Rhodobacter sphaeroides: quantum efficiency and X-ray structure. Biochemistry 44: 6920–6928

    Article  PubMed  CAS  Google Scholar 

  • Prisner TF, van der Est A, Bittl R, Lubitz W, Stehlik D and Möbius K (1995) Time-resolved W-band (95 GHz) EPR spectroscopy of Zn-substituted reaction centers of Rhodobacter sphaeroides R-26. Chem Phys 194: 361–370

    Article  CAS  Google Scholar 

  • Prisner T, Rohrer M and MacMillan F (2001) Pulsed EPR spectroscopy: biological applications. Annu Rev Phys Chem 52: 279–313

    Article  PubMed  CAS  Google Scholar 

  • Pushkar YN, Zech SG, Stehlik D, Brown S, van der Est A, and Zimmermann H (2002) Orientation and protein–cofactor interactions of monosubstituted n-alkyl naphthoquinones in the A binding site of photosystem I. J Phys Chem B 106: 12052–12058.

    Article  CAS  Google Scholar 

  • Pushkar YN, Golbeck JH, Stehlik D and Zimmermann H (2004a) Asymmetric hydrogen-bonding of the quinone cofactor in Photosystem I probed by 13C labeled naphthoquinones. J Phys Chem B 108: 9439–9450

    Article  CAS  Google Scholar 

  • Pushkar YN, Stehlik D, van Gastel M and Lubitz W (2004b) An EPR/ENDOR study of the asymmetric hydrogen bond between the quinone electron acceptor and the protein backbone in Photosystem I. J Mol Struct 700: 233–241

    Article  CAS  Google Scholar 

  • Pushkar YN, Karyagina I, Stehlik D, Brown S and van der Est A (2005) Recruitment of a foreign quinone into the A site of photosystem I. Consecutive forward electron transfer from A0 to A to FX with anthraquinone in the A site as studied by transient EPR. J Biol Chem 280: 12382–12390

    Article  PubMed  CAS  Google Scholar 

  • Reiter RC, Stevenson GR and Wang ZY (1990) Total π spin density mapping as a function of hydrogen bonding involving an anion radical. J Phys Chem 94: 5717–5720

    Article  CAS  Google Scholar 

  • Rigby SEJ, Muhiuddin IP, Evans MCW, Purton S and Heathcote P (2002) Photoaccumulation of the PsaB phyllosemiquinone in Photosystem I of Chlamydomonas reinhardtii. Biochim Biophys Acta 1556: 13–20

    Article  PubMed  CAS  Google Scholar 

  • Rutherford AW and Sétif P (1990) Orientation of P700, the primary electron donor of photosystem I. Biochim Biophys Acta 1019: 128–132

    Article  CAS  Google Scholar 

  • Sakuragi Y, Zybailov B, Shen GZ, Jones AD, Chitnis PR, van der Est A, Bittl R, Zech S, Stehlik D, Golbeck JH and Bryant DA (2002) Insertional inactivation of the menG gene, encoding 2-phytyl-1,4-naphthoquinone methyltransferase of Synechocystis sp PCC 6803, results in the incorporation of 2-phytyl-1,4-naphthoquinone into the A site and alteration of the equilibrium constant between A and FX in Photosystem I. Biochemistry 41: 394–405

    Article  PubMed  CAS  Google Scholar 

  • Sakuragi Y, Zybailov B, Shen G, Bryant DA, Golbeck JH, Diner BA, Karyagina I, Pushkar Y and Stehlik D (2005) Characterization of a foreign quinone into the A site of photosystem I. Characterization of a menB rubA double deletion mutant in Synechococcus sp. PCC 7002 devoid of FX, FA and FB and containing plastoquinone or exchanged 9,10-anthraquinone. J Biol Chem 280: 12371–12381

    Article  PubMed  CAS  Google Scholar 

  • Salikhov KM, Bock CH and Stehlik D (1990) Time development of electron spin polarization in magnetically coupled, spin correlated radical pairs. Appl Magn Reson 1: 195–211

    Article  CAS  Google Scholar 

  • Salikhov KM, van der Est AJ and Stehlik D (1999) The transient EPR spectra and spin dynamics of coupled three-spin systems in photosynthetic reaction centres. Appl Mag Res 16: 101–134

    CAS  Google Scholar 

  • Salikhov KM, Zech SG and Stehlik D (2002a) Light induced radical pair intermediates in photosynthetic reaction centres in contact with an observer spin label: spin dynamics and effects on transient EPR spectra. Mol Phys 100: 1311–1321

    Article  CAS  Google Scholar 

  • Salikhov KM, Zech SG and Stehlik D (2002b) Light induced radical pair intermediates in photosynthetic reaction centres in contact with an observer spin label: spin dynamics and effects on transient EPR spectra. Mol Phys 100: 3331–3331

    Article  CAS  Google Scholar 

  • Salikhov KM, Pushkar YN, Golbeck JH and Stehlik D (2003) Interpretation of multifrequency transient EPR spectra of the P700 +A0QK - state in Photosystem I complex with a sequential correlated radical pair model: wild type versus A0 mutants. Appl Magn Reson 24: 467–482

    CAS  Google Scholar 

  • Santabarbara S, Kuprov I, Fairclough WV, Purton S, Hore P, Heathcote P and Evans MC (2005) Bidirectional electron transfer in Photosystem I: determination of two distances between P700 + and A1 - in spin-correlated radical pairs. Biochemistry 44: 2119–2128

    Article  PubMed  CAS  Google Scholar 

  • Schlodder E, Falkenberg K, Gergeleit M and Brettel K (1998) Temperature dependence of forward and reverse electron transfer from A1 -, the reduced secondary electron acceptor in photosystem I. Biochemistry 37: 9466–9476

    Article  PubMed  CAS  Google Scholar 

  • Schweiger A and Jeschke G (2001) Principles of Pulse Electron Paramagnetic Resonance Spectroscopy. Oxford University Press, Oxford

    Google Scholar 

  • Semenov AY, Vassiliev IR, van der Est A, Mamedov MD, Zybailov B, Shen GZ, Stehlik D, Diner BA, Chitnis PR and Golbeck JH (2000) Recruitment of a foreign quinone into the A site of Photosystem I –altered kinetics of electron transfer in phylloquinone biosynthetic pathway mutants studied by time-resolved optical, EPR, and electrometric techniques. J Biol Chem 275: 23429–23438

    Article  PubMed  CAS  Google Scholar 

  • Sieckmann I, Brettel K, Bock C, van der Est A and Stehlik D (1993) Transient electron-paramagnetic-resonance of the triplet-state of P700 in Photosystem I. Evidence for triplet delocalization at room-temperature. Biochemistry 32: 4842–4847

    Article  PubMed  CAS  Google Scholar 

  • Stehlik D and Möbius K (1997) New EPR methods for investigating photoprocesses with paramagnetic intermediates. Annu Rev Phys Chem 48: 745–784

    Article  PubMed  CAS  Google Scholar 

  • Stehlik D, Bock CH and Thurnauer MC (1989) Transient EPR-spectroscopy of photoinduced electronic spin states in rigid matrices. In: Hoff AJ (ed) Advanced EPR in Biology and Biochemistry, pp 371–403. Elsevier, Amsterdam

    Google Scholar 

  • Stone A (1963) Gauge invariance of the g tensor. Proc R Soc Lond Ser A 271: 424

    Google Scholar 

  • Tang J, Utschig LM, Poluektov O and Thurnauer MC (1999) Transient W-band EPR study of sequential electron transfer in photosynthetic bacterial reaction centers. J Phys Chem B 103: 5145–5150

    Article  CAS  Google Scholar 

  • Thurnauer MC and Norris JR (1980) An electron-spin echo phase-shift observed in photosynthetic algae –possible evidence for dynamic radical pair interactions. Chem Phys Lett 76: 557–561

    Article  CAS  Google Scholar 

  • van der Est A (2001) Light-induced spin polarization in type I photosynthetic reaction centres. Biochim Biophys Acta 1507: 212–225

    Article  PubMed  Google Scholar 

  • van der Est A, Sieckmann I, Lubitz W and Stehlik D (1995) Differences in the binding of the primary quinone acceptor in Photosystem-I and reaction centers of Rhodobacter sphaeroides- R26 studied with transient EPR spectroscopy. Chem Phys 194: 349–359

    Article  Google Scholar 

  • Xu W, Chitnis P, Valieva A, van der Est A, Pushkar YN, Krzystyniak M, Teutloff C, Zech SG, Bittl R, Stehlik D, Zybailov B, Shen GZ and Golbeck JH (2003a) Electron transfer in cyanobacterial Photosystem I –I. Physiological and spectroscopic characterization of site-directed mutants in a putative electron transfer pathway from A0 through A to FX. J Biol Chem 278: 27864–27875

    Article  CAS  Google Scholar 

  • Xu W, Chitnis PR, Valieva A, van der Est A, Brettel K, Guergova-Kuras M, Pushkar YN, Zech SG, Stehlik D, Shen GZ, Zybailov B and Golbeck JH (2003b) Electron transfer in cyanobacterial photosystem I –II. Determination of forward electron transfer rates of site-directed mutants in a putative electron transfer pathway from A0 through A to FX. J Biol Chem 278: 27876–27887

    Article  CAS  Google Scholar 

  • Zech SG, Hofbauer W, Kamlowski A, Fromme P, Stehlik D, Lubitz W and Bittl R (2000) A structural model for the charge separated state P700 +A1 - in photosystem I from the orientation of the magnetic interaction tensors. J Phys Chem B 104: 9728–9739

    Article  CAS  Google Scholar 

  • Zeilinger A (1999) Experiment and the foundations of quantum physics. Rev Modern Phys 71: 288–297

    Article  Google Scholar 

  • Zouni A, Witt HT, Kern J, Fromme P, Krauß N, Saenger W and Orth P (2001) Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Ångstrom resolution. Nature 409: 739–743

    Article  PubMed  CAS  Google Scholar 

  • Zouni A, Biesiadka J, Loll B, Kern J and Irrgang K (2004) Crystal structure of cyanobacterial photosystem II at 3.2 Å resolution: a closer look at the Mn cluster. Phys Chem Chem Phys 6: 4733–4736

    Google Scholar 

  • Zybailov B, van der Est A, Zech SG, Teutloff C, Johnson TW, Shen GZ, Bittl R, Stehlik D, Chitnis PR and Golbeck JH (2000) Recruitment of a foreign quinone into the A site of photosystem I –II. Structural and functional characterization of phylloquinone biosynthetic pathway mutants by electron paramagnetic resonance and electron-nuclear double resonance spectroscopy. J Biol Chem 275: 8531–8539

    Article  PubMed  CAS  Google Scholar 

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  1. Fachbereich Physik, Freie Universität Berlin, Arnimallee 14, Berlin, D-14195, Germany

    Dietmar Stehlik

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  1. The Pennsylvania State University, Pennsylvania, USA

    John H. Golbeck

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Stehlik, D. (2006). Transient EPR Spectroscopy as Applied to Light-Induced Functional Intermediates Along the Electron Transfer Pathway in Photosystem I. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_23

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