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Confocal and Transmission Electron Microscopy for Plant Studies

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Advances in Plant Ecophysiology Techniques

Abstract

The development of high-sensitive microscopic techniques, together with the development of methods for the conservation of cells and the improvement of methods for obtaining ultra-fine sections, gave rise to the knowledge of the cellular structure in the second half of the twentieth century. The combination of these techniques with immune-cytological techniques and the use of computational systems of image analysis have increased the interest in the use of electron and confocal microscopy in biological research. Knowing which areas of the cell morphology are being altered after treatment is essential to establish new physiological or biochemical measures for the in detail study of the effects of stressing factors on plants. A general view of the root or shoot tissues examined under microscopy will allow the identification of cellular organization, where alterations can be detected as erroneous division patterns, loss of cell identity or cellular disorganization (Rost et al. 1996; Zhu and Rost 2000). In particular, root tips act as finely tuned sensor for different kinds of stress, which makes their study especially interesting for interpreting the plant stress response.

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Acknowledgments

The implementation of these techniques was possible thanks to the invaluable assistance of Inés Pazos and Jesús Méndez from the Central Research Services (CACTI) of the University of Vigo.

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Authors and Affiliations

  1. Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Adela M. Sánchez-Moreiras

  2. Department of Biology, Ecology and Earth Science, University of Calabria, Arcavacata di Rende, CS, Italy

    Marianna Pacenza & Leonardo Bruno

  3. Department Agraria, Mediterranea University, Reggio Calabria, Italy

    Fabrizio Araniti

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  1. Adela M. Sánchez-Moreiras
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  2. Marianna Pacenza
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  3. Fabrizio Araniti
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Correspondence to Leonardo Bruno .

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Editors and Affiliations

  1. Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Adela M. Sánchez-Moreiras

  2. Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Manuel J. Reigosa

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Sánchez-Moreiras, A.M., Pacenza, M., Araniti, F., Bruno, L. (2018). Confocal and Transmission Electron Microscopy for Plant Studies. In: Sánchez-Moreiras, A., Reigosa, M. (eds) Advances in Plant Ecophysiology Techniques. Springer, Cham. https://doi.org/10.1007/978-3-319-93233-0_15

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