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Pinus radiata (D. Don) Somatic Embryogenesis

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Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 84))

Abstract

Radiata pine ( Pinus radiata D. Don) is one of the most widely planted exotic pine species in rainfall environments of the Southern hemisphere (Yan et al. 2006). Its fast growth has stimulated an exhaustive study of wood production, and the development of breeding programs (Espinel et al. 1995; Codesido and Fernández-López 2009). Although utility of in vitro organogenesis has been proven for clonal propagation of this species (Aitken-Christie et al. 1985), a limitation of this method is the high cost of the process for mass production commercially.

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References

  • Aitken-Christie J, Singh AP, Davies H (1988) Multiplication of meristematic tissue: a new tissue culture system for radiata pine. In: Hanover JW, Keathley DE (eds) Genetic manipulation of woody plants. Plenum Publishing Corp, New York, pp 413–432

    Chapter  Google Scholar 

  • Aitken-Christie J, Singh AP, Horgan KJ, Thorpe T (1985) Explant developmental state and shoot formation in Pinus radiata cotyledons. Bot Gaz 146:190–203

    Article  Google Scholar 

  • Aquea F, Arce-Johnson P (2008) Identification of genes expressed during early somatic embryogenesis in Pinus radiata. Plant Physiol Biochem 46(5–6):559–568

    Article  CAS  PubMed  Google Scholar 

  • Arakawa T, Carpenter JF, Kita YA, Crowe JH (1990) The basis for toxicity of certain cryoprotectans-a hypothesis. Cryobiol 27:401–415

    Article  CAS  Google Scholar 

  • Barra-Jiménez A, Aronen TS, Alegre J, Toribio M (2015) Cryopreservation of embryogenic tissues from mature holm oak trees. Cryobiol 70(3):217–225

    Article  CAS  Google Scholar 

  • Bomal C, Tremblay FM (2000) Dried cryopreserved somatic embryos of two Picea species provide suitable material for direct plantlet regeneration and germplasm storage. Ann Bot 86:177–183

    Article  Google Scholar 

  • Breton D, Harvengt L, Trontin JF, Bouvet A, Favre JM (2006) Long-term subculture randomly affects morphology and subsequent maturation of early somatic embryos in maritime pine. Plant Cell Tiss Org Cult 87:95–108

    Article  Google Scholar 

  • Codesido V, Fernández-López J (2009) Juvenile radiata pine clonal seed orchard management in Galicia (NW Spain). Eur J For Res 133(1):177–190

    Article  Google Scholar 

  • Espinel S, Aragonés A, Ritter E (1995) Performance of different provenances and of the local-population of the Monterrey pine (Pinus radiata D.-Don) in Northern Spain. Ann Sci For 52(5):515–519

    Article  Google Scholar 

  • Fehér A (2015) Somatic embryogenesis—stress-induced remodeling of plant cell fate. Biochim Biophys Acta 1849:385–402

    Article  CAS  PubMed  Google Scholar 

  • Gale S, John A, Benson EE (2007) Cryopreservation of Picea sitchensis (sitka spruce) embryogenic suspensor masses. Cryo Lett. 28:225–239

    Google Scholar 

  • García-Mendiguren O, Montalbán IA, Goicoa T, Ugarte MD, Moncaleán P (2016) Environmental conditions at the initial stages of Pinus radiata somatic embryogenesis affect the production of somatic embryos. Trees-Struct Funct 30(3):949–958

    Article  CAS  Google Scholar 

  • García-Mendiguren O, Montalbán IA, Stewart D, Klimaszewska K, Moncaleán P, Rutledge B (2015) Gene expression profiling of shoot-derived calli from adult radiata pine and zygotic embryo-derived embryonal masses. PLoS ONE 10–6:1–19

    Google Scholar 

  • Hargreaves CL, Grace LJ, Holden DG (2002) Nurse culture for efficient recovery of cryopreserved Pinus radiata D. Don embryogenic cell lines. Plant Cell Rep 21:40–45

    Article  CAS  Google Scholar 

  • Hargreaves C, Grace L, van der Mass S, Reeves C, Holden G, Menzies M, Kumar S, Foggo M (2004) Cryopreservation of Pinus radiata zygotic embryo cotyledons: effect of storage duration on adventitious shoot formation and plant growth after 2 years in the field. Can J For Res 34(3):600–608

    Article  Google Scholar 

  • Hargreaves CL, Reeves CB, Find JI, Gough K, Josekutty P, Skudder DB, Van der Maas SA, Sigley MR, Menzies MI, Low CB, Mullin TJ (2009) Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation. Can J For Res 39:1566–1574

    Article  Google Scholar 

  • Krajnakova J, Sutela S, Aronen T, Gomory D, Vianello A, Haggman H (2011) Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity. Cryobiol 63:17–25

    Article  CAS  Google Scholar 

  • Klimaszewska K, Bernier-Cardou M, Cyr DR, Sutton BCS (2000) Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L. In Vitro Cell Dev Biol Plant 36:279–286

    Article  CAS  Google Scholar 

  • Kong L, von Aderkas P (2011) A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer. Plant Cell Tiss Org Cult 206(1):115–125

    Article  CAS  Google Scholar 

  • Kvaalen H, Johnsen O (2007) Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis. New Phytol 177:49–59

    PubMed  Google Scholar 

  • Lelu-Walter MA, Bernier-Cardou M, Klimaszewska K (2008) Clonal plant production from self- and cross-pollinated seed families of Pinus sylvestris (L.) through somatic embryogenesis. Plant Cell Tiss Org Cult 92:31–45

    Article  Google Scholar 

  • Montalbán IA, De Diego N, Moncaleán P (2010) Bottlenecks in Pinus radiata somatic embryogenesis: improving maturation and germination. Trees-Struct Funct 24:1061–1071

    Article  Google Scholar 

  • Montalbán IA, De Diego N, Moncaleán P (2012) Enhancing initiation and proliferation in radiata pine (Pinus radiata D. Don) somatic embryogenesis through seed family screening, zygotic embryo staging and media adjustments. Acta Physiol Plant 34:451–460

    Article  CAS  Google Scholar 

  • Montalbán IA, Novák O, Rolčik J, Strnad M, Moncaleán P (2013) Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees. Physiol Plant 148:214–231

    Article  CAS  PubMed  Google Scholar 

  • Montalbán IA, García-Mendiguren O, Goicoa T, Ugarte MD, Moncaleán P (2015) Cold storage of initial plant material affects positively somatic embryogenesis in Pinus radiata. New Forest 46:309–317

    Article  Google Scholar 

  • Morel A, Teyssier C, Trontin J-F, Eliášová K, Pešek B, Beaufour M, Morabito D, Boizot N, Le Metté C, Belal-Bessai L, Reymond I, Harvengt L, Cadene M, Corbineau F, Vágner M, Label P, Lelu- Walter M-A (2014) Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses. Physiol Plant 152:184–201

    Article  CAS  PubMed  Google Scholar 

  • Neilson KA, Gammulla CG, Mirzaei M, Imin N, Haynes PA (2010) Proteomic analysis of temperature stress in plants. Proteomics 10:828–845

    Article  CAS  PubMed  Google Scholar 

  • Park YS (2002) Implementation of conifer somatic embryogenesis in clonal forestry: technical requirements and deployment considerations. Ann Forest Sci 59:651–656

    Article  Google Scholar 

  • Pullman GS, Gupta PK, Timmis R, Carpenter C, Kreitinger M, Welty E (2005) Improved Norway spruce somatic embryo development through the use of abscisic acid combined with activated carbon. Plant Cell Rep 24(5):271–279

    Article  CAS  PubMed  Google Scholar 

  • Quoirin M, Lepoivre P (1977) Études des milieux adaptés aux cultures in vitro de Prunus. Acta Hort 78:437–442

    Article  Google Scholar 

  • Salaj T, Matusíková I, Swennen R, Panis B, Salaj J (2012) Long-term maintenance of Pinus nigra embryogenic cultures through cryopreservation. Acta Physiol Plant 34:227–233

    Article  Google Scholar 

  • Smith DR, Walter C, Warr AA, Hargreaves CL, Grace LJ (1994) Somatic embryogenesis joins the plantation forestry revolution in New Zealand. In: Biological sciences symposium, TAPPI Proceedings, Minneapolis, USA, pp 19–29

    Google Scholar 

  • Teyssier C, Grondin C, Bonhomme L, Lomenech AM, Vallance M, Morabito D, Label P, Lelu-Walter MA (2011) Increased gelling agent concentration promotes somatic embryo maturation in hybrid larch (Larix X eurolepsis): a 2-DE proteomic analysis. Physiol Plant 141:152–165

    Article  CAS  PubMed  Google Scholar 

  • Von Aderkas P, Bonga JM (2000) Influencing micropropagation and somatic embryogenesis in mature trees by manipulation of phase change, stress and culture environment. Tree Physiol 20:921–928

    Article  Google Scholar 

  • Walter C, Find JI, Grace LJ (2005) Somatic embryogenesis and genetic transformation in Pinus radiata. In: Jain SM, Gupta PK (eds), Protocol for somatic embryogenesis in woody plants. Forestry sciences, vol 77. Springer, Dordrecht, pp 491–504

    Google Scholar 

  • Yan H, Bi HQ, Li RW, Eldridge R, Wu ZX, Li Y, Simpson J (2006) Assessing climatic suitability of Pinus radiata (D. Don) for summer rainfall environment of southwest China. Forest Ecol Manag 234(1–3):199–208

    Article  Google Scholar 

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Acknowledgements

This research was funded by MINECO (Spanish Government) project (AGL2013-4700-C4-2R; AGL2016-76143-C4-3R) and DECO (Basque Government). Thanks to CYTED (Programa iberoamericano de Ciencia y Tecnología para el desarrollo) for founding BIOALI net and make possible the establishment of successful international collaborations.

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Authors and Affiliations

  1. Department of Plant Production, Centro de Arkaute, Neiker-Tecnalia, 01080, Vitoria-Gasteiz, Alava, Spain

    Itziar A. Montalbán & Paloma Moncaleán

Authors
  1. Itziar A. Montalbán
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  2. Paloma Moncaleán
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Correspondence to Paloma Moncaleán .

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Editors and Affiliations

  1. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Shri Mohan Jain

  2. Technology Center, Weyerhaeuser Company, Federal Way, Washington, USA

    Pramod Gupta

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Montalbán, I.A., Moncaleán, P. (2018). Pinus radiata (D. Don) Somatic Embryogenesis. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_1

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