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Transcriptomic Approaches for Muscle Biology and Disorders

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Omics Approaches to Understanding Muscle Biology

Part of the book series: Methods in Physiology ((METHPHYS))

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Abstract

Transcriptomics approaches have been advancing quickly in the past 20 years. Technologies including various arrays and sequencing technologies are used to determine expression of RNA transcripts in cells and tissues. While the earlier approaches focus on the messenger RNA (mRNA), all other types of RNA transcripts, such as micro RNAs and non-coding RNAs, can be easily studied using current approaches. For skeletal muscle research, investigators use transcriptomics approaches to study basic muscle biology; molecular responses to physiological and environmental stimuli; effects of aging on muscles; disease mechanism of muscle disorders; molecular changes in muscles of non-muscle diseases; and molecular responses to therapeutic. This chapter focuses on current approaches and platforms used in the studies. Platform selections and limitations as well as data analysis will be discussed. Emerging approaches such as single cell profiling, single nucleus profiling, modified RNA profiling, and spatial transcription profiling are described in the chapter.

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Authors and Affiliations

  1. DNA Sequencing and Genomics Core, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Poching Liu

  2. Center for Genetic Medicine Research, Children’s National Medical Center, Washington, DC, USA

    Surajit Bhattacharya

  3. Center for Genetic Medicine Research, Children’s National Health System, Children’s Research Institute, Washington, DC, USA

    Yi-Wen Chen

  4. Department of Genomics and Precision Medicine, School of Medicine and Health Science, George Washington University, Washington, DC, USA

    Yi-Wen Chen

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  1. Poching Liu
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  2. Surajit Bhattacharya
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  3. Yi-Wen Chen
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Correspondence to Yi-Wen Chen .

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Editors and Affiliations

  1. Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, Merseyside, UK

    Jatin George Burniston

  2. Children’s National Health System, George Washington University, Washington, DC, USA

    Yi-Wen Chen

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Liu, P., Bhattacharya, S., Chen, YW. (2019). Transcriptomic Approaches for Muscle Biology and Disorders. In: Burniston, J., Chen, YW. (eds) Omics Approaches to Understanding Muscle Biology. Methods in Physiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9802-9_5

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