Overview
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 373)
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About this book
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.
Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
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Keywords
Table of contents (14 protocols)
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Front Matter
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Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: Pyrosequencing Protocols
Editors: John M. Walker, Sharon Marsh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1597453773
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-645-0Published: 21 November 2006
Softcover ISBN: 978-1-61737-693-1Published: 01 December 2010
eBook ISBN: 978-1-59745-377-6Published: 04 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 196
Topics: Biochemistry, general, Human Genetics