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The Proteomics Protocols Handbook

  • John M. Walker

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Brian Berg Vandahl, Gunna Christiansen, Svend Birkelund
    Pages 19-26
  3. Joakim Norbeck
    Pages 27-30
  4. Melinda L. Ramsby, Gregory S. Makowski
    Pages 37-48
  5. Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari, Craig D. Shriver
    Pages 49-54
  6. Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony G. Sullivan, Henry Brzeski, Richard I. Somiari et al.
    Pages 59-66
  7. William L. Godfrey, Colette J. Rudd, Sujata Iyer, Diether Recktenwald
    Pages 67-78
  8. Régis Dieckmann, Yohann Couté, Denis Hochstrasser, Jean-Jacques Diaz, Jean-Charles Sanchez
    Pages 79-85
  9. Henry Brzeski, Stephen Russell, Anthony G. Sullivan, Richard I. Somiari, Craig D. Shriver
    Pages 119-131
  10. Michael Fountoulakis
    Pages 133-144
  11. Masamichi Oh-Ishi, Tadakazu Maeda
    Pages 145-149
  12. Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal, Christopher M. Taylor
    Pages 167-175
  13. Julia Poland, Thierry Rabilloud, Pranav Sinha
    Pages 177-184
  14. Carlos Fernandez-Patron
    Pages 185-192
  15. Thomas H. Steinberg, Courtenay R. Hart, Wayne F. Patton
    Pages 215-222
  16. Richard I. Somiari, Stephen Russell, Stella B. Somiari, Anthony G. Sullivan, Darrell L. Ellsworth, Henry Brzeski et al.
    Pages 223-237
  17. Françoise Seillier-Moiseiwitsch
    Pages 239-258
  18. Christine Hoogland, Khaled Mostaguir, Ron D. Appel
    Pages 259-266
  19. Patricia M. Palagi, Daniel Walther, Gérard Bouchet, Sonja Voordijk, Ron D. Appel
    Pages 267-277
  20. Peter F. Lemkin, Gregory C. Thornwall, Jai Evans
    Pages 279-305
  21. Michele Learmonth, Alastair Aitken
    Pages 311-314
  22. Alastair Aitken
    Pages 319-324
  23. Patrick W. Cooley, Janice L. Joss, Femia G. Hopwood, Nichole L. Wilson, Andrew A. Gooley
    Pages 341-354
  24. Francesco Giorgianni, Sarka Beranova-Giorgianni
    Pages 367-374
  25. Eugene Yi, Hui Zhang, Kelly Cooke, Ruedi Aebersold, David R. Goodlett
    Pages 385-392
  26. Angela K. Walker, Gary Rymar, Philip C. Andrews
    Pages 417-429
  27. Franz-Georg Hanisch, Stefan Müller
    Pages 439-457
  28. Biju Issac, Gajendra P. S. Raghava
    Pages 503-525
  29. Chris P. Ponting, Ewan Birney
    Pages 527-541

About this book

Introduction

Proteomics methodology has revolutionized the way that proteins are studied and opened new channels to understanding both cell functions and the cellular changes involved in disease states. In The Proteomics Protocols Handbook, hands-on researchers describe in step-by-step detail a wide range of proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques (73 in all) address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and posttranslational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein, including novel mass spectrometry and LC-MS techniques, protein array technology, and a variety of structural and functional proteomics techniques needed to determine of the function of newly discovered protein sequences. The use of X-ray crystallography and NMR to determine protein structure is also described. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, The Proteomics Protocols Handbook offers molecular biologists, protein chemists, clinical and medicinal chemists, biochemists, and microbiologists a comprehensive collection of today's best tools for understanding not only the function of individual proteins in the cell, but also for identifying new therapeutic targets.

Keywords

Proteomics biology membrane proteins molecular biology phosphorylation protein translation

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information