The Proteomics Protocols Handbook

  • John M. Walker

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Brian Berg Vandahl, Gunna Christiansen, Svend Birkelund
    Pages 19-26
  3. Joakim Norbeck
    Pages 27-30
  4. Melinda L. Ramsby, Gregory S. Makowski
    Pages 37-48
  5. Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari, Craig D. Shriver
    Pages 49-54
  6. Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony G. Sullivan, Henry Brzeski, Richard I. Somiari et al.
    Pages 59-66
  7. William L. Godfrey, Colette J. Rudd, Sujata Iyer, Diether Recktenwald
    Pages 67-78
  8. Régis Dieckmann, Yohann Couté, Denis Hochstrasser, Jean-Jacques Diaz, Jean-Charles Sanchez
    Pages 79-85
  9. Henry Brzeski, Stephen Russell, Anthony G. Sullivan, Richard I. Somiari, Craig D. Shriver
    Pages 119-131
  10. Michael Fountoulakis
    Pages 133-144
  11. Masamichi Oh-Ishi, Tadakazu Maeda
    Pages 145-149
  12. Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal, Christopher M. Taylor
    Pages 167-175
  13. Julia Poland, Thierry Rabilloud, Pranav Sinha
    Pages 177-184
  14. Carlos Fernandez-Patron
    Pages 185-192

About this book


Proteomics methodology has revolutionized the way that proteins are studied and opened new channels to understanding both cell functions and the cellular changes involved in disease states. In The Proteomics Protocols Handbook, hands-on researchers describe in step-by-step detail a wide range of proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques (73 in all) address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and posttranslational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein, including novel mass spectrometry and LC-MS techniques, protein array technology, and a variety of structural and functional proteomics techniques needed to determine of the function of newly discovered protein sequences. The use of X-ray crystallography and NMR to determine protein structure is also described. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, The Proteomics Protocols Handbook offers molecular biologists, protein chemists, clinical and medicinal chemists, biochemists, and microbiologists a comprehensive collection of today's best tools for understanding not only the function of individual proteins in the cell, but also for identifying new therapeutic targets.


Proteomics biology membrane proteins molecular biology phosphorylation protein translation

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-343-5
  • Online ISBN 978-1-59259-890-8
  • About this book