Abstract
Differential screening of entire cell proteomes by two-dimensional gel electrophoresis (2-DE) often leads to the identification of several differentially expressed but functionally unrelated target proteins. Hence, this approach often fails to give a comprehensive picture of the underlying biological mechanism. Thus, the level of complexity of the entire cell proteome needs to be reduced (1). Cell fractionation is both a way of reducing complexity and a way to target a specific cellular compartment. The purification of nucleoli from three Hodgkin nonadherent lymphoma cell lines is presented here. It is based on an original method from Muramatsu and Onishi (2) and has been adapted from a protocol developed for HeLa cells (3).
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© 2005 Humana Press Inc., Totowa, NJ
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Dieckmann, R., Couté, Y., Hochstrasser, D., Diaz, JJ., Sanchez, JC. (2005). Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separation. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-890-0:079
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DOI: https://doi.org/10.1385/1-59259-890-0:079
Publisher Name: Humana Press
Print ISBN: 978-1-58829-343-5
Online ISBN: 978-1-59259-890-8
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