Overview
- Editors:
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Antonio Giordano
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Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia
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Gaetano Romano
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Department of Neurosurgery, Thomas Jefferson University, Philadelphia
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Table of contents (22 protocols)
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Cellular Response to Deoxyribonucleic Acid Damage
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- David P. Turner, Anthony T. Yeung, Alfonso Bellacosa
Pages 133-137
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Retroviridae-Based Vectors: Protocols for Lentiviral- and Retroviral-Mediated Gene Transfer to Engineer Cell Culture Systems
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Front Matter
Pages 139-139
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- Tiziana Tonini, Pier Paolo Claudio, Antonio Giordano, Gaetano Romano
Pages 141-148
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- Tiziana Tonini, Pier Paolo Claudio, Antonio Giordano, Gaetano Romano
Pages 149-153
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- Tiziana Tonini, Pier Paolo Claudio, Antonio Giordano, Gaetano Romano
Pages 155-157
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Detection of Gene Expression in Subcellular Compartments
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Front Matter
Pages 159-159
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- Nicoletta Zini, Liliana Solimando, Caterina Cinti, Nadir Mario Maraldi
Pages 161-169
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- Massimo Riccio, Maja Dembic, Caterina Cinti, Spartaco Santi
Pages 171-177
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Back Matter
Pages 179-185
About this book
Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protocols available, along with commercial kits and reagents, a variety of the more common techniques have not been included in our book. The protocols described, based on rather sophisticated techniques for in vivo and in vitro studies, consist of molecular biology, biochemistry, and various types of immunoassays. Indeed, the authors have successfully accomplished an arduous task by presenting several topics in the simplest possible manner. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.
Reviews
"State-of-the-art and highly practical...offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies." - Tumori
Editors and Affiliations
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Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia
Antonio Giordano
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Department of Neurosurgery, Thomas Jefferson University, Philadelphia
Gaetano Romano