Overview
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 177)
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Table of contents (20 protocols)
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General Methods
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Applications
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Alternative Strategies
About this book
Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
Reviews
"The stated goal of the volume is "to introduce the yeast two-hybrid systems to students, research assistants, research associates, and other more senior investigators considering this as a new approach." This goal is well achieved as the volume is easy to read and comprehensive.... the first section of this volume provides in depth detailing of yeast protocols, including several very useful trouble shooting solutions and tips well beyond the scope of those found in the instruction manuals accompanying commercial kits....this book is a worthwhile investment to ensure that the most is gained from your two-hybrid experiments and thus I would recommend it." - Molecular Biotechnology
"This current volume is a practical, laboratory manual with detailed methods and trouble-shooting tips for the yeast two-hybrid approach. The volume includes a brief historical overview, with chapters addressing yeast husbandry and media preparation and yeast genetic modification. The manual lso includes detailed accountsof how to construct a two-hybrid screen and how to analyse the positives. I found the book easy to read, and the protocols were clear and straightforward to follow. There are a number of useful tables, including yeast genotypes and genetic markers as well as available vectors. I would recommend this book as a practical laboratory manual for anyone contemplating a search for a partner protein using the yeast two-hybrid approach." -The Endocrinologist
Editors and Affiliations
Bibliographic Information
Book Title: Two-Hybrid Systems
Book Subtitle: Methods and Protocols
Editors: Paul N. MacDonald
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592592104
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2001
Hardcover ISBN: 978-0-89603-832-5Published: 17 May 2001
Softcover ISBN: 978-0-89603-808-0Published: 17 May 2001
eBook ISBN: 978-1-59259-210-4Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 336
Topics: Cell Biology