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Helicobacter pylori Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Christopher L. Clayton

    1. Glaxo Wellcome Research and Development Ltd., Stevenage, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Harry L. T. Mobley

    1. University of Maryland School of Medicine, Baltimore

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 8)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Introduction: Medical Significance of H. pylori

    • Martin J. Blaser
    Pages 1-6

  3. Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture

    • Stewart Goodwin
    Pages 7-18

  4. Serology and Urea Breath Test in the Diagnosis of H. pylori Infection

    • Peter R. Hawtin
    Pages 19-29

  5. The Detection of H. pylori by the Polymerase Chain Reaction

    • Nicholas P. Mapstone
    Pages 31-36

  6. PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples

    • Stephen B. Fritz, T. Ulf Westblom
    Pages 37-40

  7. In vitro Susceptibility Testing of H. pylori

    • Alistair McLaren
    Pages 41-51

  8. Defined Media for H. pylori

    • David J. Reynolds
    Pages 53-61

  9. Studies of Respiration in H. pylori

    • Roger J. Miles, Huai-Tzu Chang
    Pages 63-68

  10. Use of NMR to Study H. pylori Metabolism

    • Peter A. Chalk, Andrew D. Roberts, Alistair A. Davison, David J. Kelly, Peter J. White
    Pages 69-80

  11. Isolation of H. pylori Genomic DNA and Restriction Analysis

    • Robert J. Owen, Jane Bickley
    Pages 81-88

  12. Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori

    • Wee Tee
    Pages 89-98

  13. Differentiation of H. pylori Strains Using PCR RFLP

    • Harry L. T. Mobley, Paul A. Foxall
    Pages 99-105

  14. PCR-DNA Sequence Typing

    • Agnès Labigne
    Pages 107-116

  15. H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method

    • Douglas E. Berg, Janaki Lelwala-Guruge, Engin T. Incecik, Kalpana Srivastava, Natalia S. Akopyants
    Pages 117-132

  16. Molecular Methods for Detecting Ulcerogenic Strains of H. pylori

    • John C. Atherton
    Pages 133-143

  17. H. pylori DNA Transformation by Natural Competence and Electroporation

    • Zhongming Ge, Diane E. Taylor
    Pages 145-152

  18. Random Mutagenesis of the H. pylori Genome

    • Agnès Labigne
    Pages 153-163

  19. Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE)

    • Nicholas Chang, Qin Jiang, Diane E. Taylor
    Pages 165-176

  20. Molecular Characterization of H. pylori Surface Antigens

    • Peter Doig, Paul W. O’Toole, Trevor J. Trust
    Pages 177-189
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Keywords

About this book

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

Reviews

"The book was designed to provide up-to-date detailed protocols for the experienced either working
in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine

Editors and Affiliations

  • Glaxo Wellcome Research and Development Ltd., Stevenage, UK

    Christopher L. Clayton

  • University of Maryland School of Medicine, Baltimore

    Harry L. T. Mobley

Bibliographic Information

  • Book Title: Helicobacter pylori Protocols

  • Editors: Christopher L. Clayton, Harry L. T. Mobley

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/0896033813

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1997

  • Hardcover ISBN: 978-0-89603-381-8Published: 24 March 1997

  • Softcover ISBN: 978-1-61737-020-5Published: 09 November 2010

  • eBook ISBN: 978-1-59259-592-1Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIII, 274

  • Topics: Biochemistry, general

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