Abstract
Helicobacter pylori has been widely studied since its discovery in 1982 by Marshall and Warren (1), but many aspects of its structure, metabolism, and physiology, including its specific growth requirements, are still largely unknown, The organism is generally grown in complex media containing tissue extracts (e.g., of brain or heart) or proteolytic enzyme digests of meat or casein supplemented with blood or serum, which has made the metabolic pathways utilized by the bacterium difficult to determine. For example, although early studies based on acid formation from sugars and detection of preformed enzymes found no evidence of saccharide fermentative pathways (2,3), more recent evidence indicates that H. pylori does indeed catabolize sugars. In a series of studies, Mendz, Hazell, and colleagues found clear evidence for the pentose phosphate pathway (4), glucokinase activity (5), and the fermentation of glucose to lactate (6).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Warren, J. R. and Marshall, B. J. (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis Lancet 1, 1273–1275
Marshall, B. J. and Warren, J. R. (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1, 1311–1315
McNulty, C. A. and Dent, J. C (1987) Rapid identification of Cumpylobacter pylori (C. pyloridis) by preformed enzymes. J. Clin. Microbiol. 25, 1683–168
Mendz, G L. and Hazell, S. L. (1991) Evidence for a pentose phosphate pathway in Helicobacter pylori. FEMS Microbiol. Lett 84, 331–336
Mendz, G. L. and Hazell, S L. (1993) Glucose phosphorylation in Helicobacter pylori. Arch Biochem. Biophys. 300, 522–525
Mendz, G. L, Hazell, S. L., and Burns, B. P. (1993) Glucose utilization and lactate production by Helicobacter pylori. J. Gen. Microbiol. 139, 3023–3028.
Sorberg, M., Nilsson, M., and Nilsson, L. E. (1993) Morphologic conversion of Helicobacter pylori from bacillary form to coccoid form evaluated by bioluminescence, microscopy and viable count. Acta Gastro-Enterol Belg. Suppl 56, 52.
Catrenich, C.E and Makin, K. M (1991) Characterization of the morphologic conversion of Helicobacter pylori from bacillary to coccoid form. Scand J Gastroenterol 26(Suppl. 181) 58–64.
Cellini, L., Allocati, N., Di Camph, E., Masulli, M., and Dainelli, B. (1993) Morphological forms in Helicobacter pylori Acta Gastro-Enterol Belg Suppl 56, 108.
Reynolds, D J and Penn, C.W. (1994) Characteristics of Helicobacter pylori growth in a defined medium and determination of its amino acid requirements. J. Gen. Microbiol. 140, 2649–2656.
Mekalanos, J. J. (1992) Environmental signals controlling expression of virulence determinants in bacteria J Bacteriol. 174, 1–7
Laemmli, U. K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Reynolds, D.J. (1997). Defined Media for H. pylori . In: Clayton, C.L., Mobley, H.L.T. (eds) Helicobacter pylori Protocols. Methods in Molecular Medicine, vol 8. Humana Press. https://doi.org/10.1385/0-89603-381-3:53
Download citation
DOI: https://doi.org/10.1385/0-89603-381-3:53
Publisher Name: Humana Press
Print ISBN: 978-0-89603-381-8
Online ISBN: 978-1-59259-592-1
eBook Packages: Springer Protocols