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  • Conference proceedings
  • Open Access
  • © 2019

Alternatives to Animal Testing

Proceedings of Asian Congress 2016

  • This is an Open Access book

  • Provides ?recent research on alternative to animal testings

  • Discusses the future approaches to alternatives in 3Rs

  • Presents the highly selected contents in the Asian Congress on Alternatives and Animal Use in the Life Sciences

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Table of contents (15 papers)

  1. Front Matter

    Pages i-vi
  2. Testing Method Development and Validation for in Vitro Skin Irritation Testing (SIT) by Using Reconstructed Human Epidermis (RhE) Skin Equivalent - EPiTRI®

    • Yu-Chun Lin, Hui-Chun Hsu, Chiu-Hsing Lin, Cheng-Yi Wu, Wannhsin Chen, Huey-Min Lai
    Pages 8-19Open Access
  3. Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)

    • Herlina B. Setijanti, Eka Rusmawati, Rahmi Fitria, Tuty Erlina, Rina Adriany, Murtiningsih
    Pages 20-32Open Access
  4. Alternative Research (3Rs) in the World, Asia and Japan

    • Tsutomu Miki Kurosawa
    Pages 33-36Open Access
  5. Approaches to Reducing Animal Use for Acute Toxicity Testing: Retrospective Analyses of Pesticide Data

    • Judy Strickland, Michael W. Paris, David Allen, Warren Casey
    Pages 37-49Open Access
  6. Cosmetic Regulation and Alternatives to Animal Experimentation in India

    • Mohammad A. Akbarsha, Benedict Mascarenhas
    Pages 57-62Open Access
  7. Guidance on the Use of Alternative Test Methods for the Safety Assessment of Cosmetics and Quasi-drugs

    • Hajime Kojima, Yoshiaki Ikarashi, Tokio Nakada, Akiko Yagami, Kenji Sugibayashi, Hiroaki Todo et al.
    Pages 63-68Open Access
  8. 3Rs in Quality Control of Human Vaccines: Opportunities and Barriers

    • Sylvie Uhlrich, Emmanuelle Coppens, Frederic Moysan, Sue Nelson, Nolwenn Nougarede
    Pages 76-82Open Access
  9. Alternative Methods for Developmental Toxicity Testing Using Mouse ESCs

    • Hee Young Kang, Eui-Bae Jeung
    Pages 105-109Open Access
  10. Futuristic Approach to Alternative Model Organisms: Hydra Stakes Its Claim

    • Anbazhagan Murugadas, Mohammed Zeeshan, Mohammad A. Akbarsha
    Pages 110-123Open Access
  11. The Lush Prize and Young Researcher Asia Awards 2016

    • Rebecca Ram
    Pages 124-128Open Access
  12. Back Matter

    Pages 129-130

About this book

This open access book presents recent advances in the pure sciences that are of significance in the quest for alternatives to the use of animals in research and describes a variety of practical applications of the three key guiding principles for the more ethical use of animals in experiments – replacement, reduction, and refinement, collectively known as the 3Rs. Important examples from across the world of implementation of the 3Rs in the testing of cosmetics, chemicals, pesticides, and biologics, including vaccines, are described, with additional information on relevant regulations. The coverage also encompasses emerging approaches to alternative tests and the 3Rs. The book is based on the most informative contributions delivered at the Asian Congress 2016 on Alternatives and Animal Use in the Life Sciences. It will be of value for those working in R&D, for graduate students, and for educators in various fields, including the pharmaceutical and cosmetic sciences, pharmacology, toxicology, and animal welfare. The free, open access distribution of Alternatives to Animal Testing is enabled by the Creative Commons Attribution license in International version 4: CC BY 4.0.


  • 3Rs for Animal experiments
  • In Vitro
  • Alternative to animal experiments
  • Toxicology
  • Animal use
  • Open Access

Editors and Affiliations

  • National Institute of Health Sciences, Kawasaki, Japan

    Hajime Kojima

  • Humane Society International, Montreal, Canada

    Troy Seidle

  • Institut für Pharmazie, Free University of Berlin, Berlin, Germany

    Horst Spielmann

About the editors

Hajime Kojima

National Institute of Health Sciences , Kawasaki, Kanagawa, Japan

Troy Seidle

Humane Society International , Montréal, QC, Canada

Horst Spielmann

Institut für Pharmazie Free University of Berlin, Berlin, Germany

Bibliographic Information

  • Book Title: Alternatives to Animal Testing

  • Book Subtitle: Proceedings of Asian Congress 2016

  • Editors: Hajime Kojima, Troy Seidle, Horst Spielmann

  • DOI:

  • Publisher: Springer Singapore

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2019

  • License: CC BY

  • Hardcover ISBN: 978-981-13-2446-8Published: 28 December 2018

  • eBook ISBN: 978-981-13-2447-5Published: 27 December 2018

  • Edition Number: 1

  • Number of Pages: VI, 130

  • Number of Illustrations: 7 b/w illustrations, 39 illustrations in colour

  • Topics: Pharmacology/Toxicology, Biochemistry, general, Animal Welfare/Animal Ethics

Buy it now

Buying options

Hardcover Book USD 59.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access