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  • Open Access
  • © 2018

Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

  • Open Access provided with a grant from the International Atomic Energy Agency

  • Provides robust, user-friendly protocols for effective pre-field screening of mutant rice for enhanced heat stress tolerance

  • Designed for use by plant breeders who need rapid screens to process large mutant populations, including segregating populations, advanced generations and germplasm collections

  • The described protocols could be adapted to screen for heat stress tolerance in other crops

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Table of contents (5 chapters)

  1. Front Matter

    Pages i-xii
  2. General Introduction

    • Fatma Sarsu
    Pages 1-7Open Access
  3. Conclusion

    • Fatma Sarsu
    Pages 33-34Open Access
  4. Back Matter

    Pages 35-39

About this book

This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly.

The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. 

Authors and Affiliations

  • Plant Breeding and Genetics Section, Joint FAO/IAEA Division, International Atomic Energy Agency, Vienna, Austria

    Fatma Sarsu

  • Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division, International Atomic Energy Agency, Vienna, Austria

    Abdelbagi M.A. Ghanim, Brian P. Forster, Ivan Ingelbrecht

  • School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

    Priyanka Das, Rajeev N. Bahuguna, Ashwani Pareek

  • Sokoine University of Agriculture, Morogoro, Tanzania

    Paul Mbogo Kusolwa

  • Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan

    Muhammed Ashraf

  • Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India

    Sneh L. Singla-Pareek

About the authors

Fatma Sarsu

Plant Breeding and Genetics Section

Joint FAO/IAEA Division

Vienna, Austria

 

Abdelbagi M.A. Ghanim

Plant Breeding and Genetics Laboratory

Joint FAO/IAEA Division

Vienna, Austria

 

Priyanka Das

School of Life Sciences

Jawaharlal Nehru University

New Delhi, India

 

Rajeev N Bahuguna

School of Life Sciences

Jawaharlal Nehru University

New Delhi, India

 

Paul Mbogo Kusolwa

Sokoine University of Agriculture

Morogoro, United Republic of Tanzania

 

Muhammed Ashraf

Nuclear Institute for Agriculture and Biology (NIAB)

Faisalabad, Pakistan

 

Sneh Lata Singla-Pareek

Plant Molecular Biology Group

International Centre for Genetic Engineering and Biotechnology

New Delhi, India

 

Ashwani Pareek

School of Life Sciences

Jawaharlal Nehru University

New Delhi, India

 

Brian P. Forster

Plant Breeding and Genetics Laboratory 

Joint FAO/IAEA Division

International Atomic Energy Agency 

Vienna, Austria



Ivan Ingelbrecht

Plant Breeding and Genetics Laboratory

Joint FAO/IAEA Division

International Atomic Energy Agency 

Vienna, Austria



Bibliographic Information

  • Book Title: Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

  • Authors: Fatma Sarsu, Abdelbagi M.A. Ghanim, Priyanka Das, Rajeev N. Bahuguna, Paul Mbogo Kusolwa, Muhammed Ashraf, Sneh L. Singla-Pareek, Ashwani Pareek, Brian P. Forster, Ivan Ingelbrecht

  • DOI: https://doi.org/10.1007/978-3-319-77338-4

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: International Atomic Energy Agency 2018

  • License: CC BY IGO

  • Hardcover ISBN: 978-3-319-77337-7Published: 23 August 2018

  • Softcover ISBN: 978-3-030-08432-5Published: 14 February 2019

  • eBook ISBN: 978-3-319-77338-4Published: 09 August 2018

  • Edition Number: 1

  • Number of Pages: XII, 39

  • Number of Illustrations: 6 b/w illustrations, 10 illustrations in colour

  • Topics: Plant Breeding/Biotechnology, Agriculture

Buy it now

Buying options

Softcover Book USD 59.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 59.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access