Proteases in Apoptosis: Pathways, Protocols and Translational Advances

This book provides a comprehensive overview of the proteases involved in programmed cell death. It presents a focused yet extensive discussion on proteolytic enzymes such as caspases, HtrAs, granzymes, calpains and cathepsins as well as laboratory protocols related to enzymology and apoptosis. Mouse model systems and non-invasive imaging techniques in apoptosis-related diseases such as cancer and neurodegeneration are also covered in this book.

While slowly unravelling the complexities of apoptosis in chapter one, the next three chapters individually elaborate on different classes of proteases that play key roles in the initiation, progression and execution of programmed cell death. The last two chapters complete this discussion by describing different laboratory methodologies and therapeutic advances involving apoptotic proteases. Protocols portraying in vitro and ex vivo colorimetric and fluorescence-based enzyme kinetic studies as well as cell death assays are explained in the fifth chapter. Preclinical in vivo models and non-invasive imaging in apoptosis to understand the complexities of disease progression and their contribution toward therapeutics is recounted in the last chapter.

The book spans topics related to both fundamental and applied biology. It would therefore be equally appealing and informative to scientists working in the field of apoptosis and those who are investigating mechanisms of proteases and enzymes in general. The protocols would certainly benefit both graduate and undergraduate students working in the related fields and provide useful leads for drug design to translational biologists involved in neurodegeneration and cancer research.

• drug design
• immune system
• mouse model
• programmed cell death
• tissue homeostasis
• Apoptosis

“This is a comprehensive overview of the mechanisms of apoptosis, with a particular emphasis on proteases. … The book is designed to inform and inspire undergraduate and graduate students to pursuing biomedical research. … it might be more appropriate for researchers in related fields who desire a comprehensive source of current information about apoptotic proteases … . This is a useful addition to the field and many will find it useful when preparing lectures or when working in related fields.” (Susan Viselli, Doody’s Book Reviews, November, 2015)

“This is a monograph on proteolysis via programmed cell death via caspase and granzyme mediated destruction in neurodegeneration. I recommend it for biochemists, cell biologists, & neurologists.” (Joseph J. Grenier, Amazon.com, November, 2015)

• Tata Memorial Centre, Integrated Biophysics and Structural Biology Lab, Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Navi Mumbai, India

  Kakoli Bose