Overview
- Details comprehensive methods to study antigen presentation by classical and non-classical MHC proteins
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 960)
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About this book
Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing events, ending with recognition of peptide-MHC complexes at the cell surface by T lymphocytes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antigen Processing: Methods and Protocols is designed for beginners and experts interested in studying antigen processing.
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Keywords
Table of contents (43 protocols)
Reviews
From the reviews:
“The book is divided into 42 chapters, each split into a short abstract, an introduction providing the relevant bibliographic overview, a detailed list of materials used in the protocol, a step-by-step description of the procedure, and a final section with notes. … Van Endert achieves what he set out to do – to provide an in-depth bench-side companion for antigen processing beginners and experts … . The book comes highly recommended.” (Clemens Hermann, Immunology News, February, 2014)
“The book is designed to be a benchside companion for researchers interested in studying antigen processing. It is intended for both beginners and experts studying antigen presentation.” (Omer Iqbal, Doody’s Book Reviews, August, 2013)Editors and Affiliations
Bibliographic Information
Book Title: Antigen Processing
Book Subtitle: Methods and Protocols
Editors: Peter Endert
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-218-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-217-9Published: 18 January 2013
Softcover ISBN: 978-1-4939-5956-3Published: 23 August 2016
eBook ISBN: 978-1-62703-218-6Published: 19 January 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 589
Number of Illustrations: 60 b/w illustrations, 25 illustrations in colour
Topics: Immunology, Antibodies