Overview
- Comprehensive guide to all techniques required for the generation of classical and conditional knockout mouse strains
- Includes techniques for ES cell establishment, ES cell in vitro differentatio, and nuclear cloning
- Provides a systematic overview of techniques for mutant phenotyping
- Covers all gene knockout techniques, including gene targeting, chemical, gene trap, and transposon mutagenesis
- Written by hands-on experts who have optimized each method for experimental success
Part of the book series: Methods in Molecular Biology (MIMB, volume 530)
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Keywords
Table of contents (25 protocols)
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Gene Modification in ES Cells
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Stem Cell Manipulation
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Genetically Engineered Mice
Reviews
From the reviews of the second edition:
"This comprehensive collection of step-by-step protocols in the field of gene targeting and mouse genetics covers such topics as gene modification in embryonic stem cells … and mutant phenotype analysis. … The book will serve as a valuable resource for researchers, scientists, and students interested in these areas. … This is a well-illustrated book … . All researchers interested in gene targeting and gene knockout protocols will find this extremely useful." (Omer Iqbal, Doody’s Review Service, September, 2009)
Bibliographic Information
Book Title: Gene Knockout Protocols
Editors: Wolfgang Wurst, Ralf Kühn
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-471-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-26-8Published: 27 March 2009
Softcover ISBN: 978-1-62703-834-8Published: 30 October 2014
eBook ISBN: 978-1-59745-471-1Published: 06 March 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 516
Topics: Biochemistry, general, Human Genetics, Animal Genetics and Genomics, Animal Models