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Characterization of Proteins

  • Felix Franks

Table of contents

  1. Front Matter
    Pages i-xxvii
  2. Felix Franks
    Pages 25-52
  3. Felix Franks
    Pages 53-94
  4. Felix Franks
    Pages 127-154
  5. P. J. Thomas
    Pages 217-232
  6. P. J. Thomas
    Pages 233-243
  7. Colin Simpson
    Pages 245-283
  8. Colin Simpson
    Pages 285-337
  9. Colin Simpson
    Pages 339-363
  10. Colin Simpson
    Pages 365-383
  11. Colin Simpson
    Pages 385-413
  12. Chester Myers
    Pages 453-466
  13. Chester Myers
    Pages 491-549
  14. Back Matter
    Pages 551-561

About this book

Introduction

Proteins are the servants of life. They occur in all component parts of living organisms and are staggering in their functional var- ty, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different proteins, fulfilling a wide range of life-supporting roles. Their production is controlled by the cell’s genetic machinery, and a malfunction of even one protein in the cell will give rise to pathological symptoms. Additions to the total number of known proteins are constantly being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation; this latter technology has become known as protein engineering. The in vivo functioning of proteins depends critically on the chemical structure of individual peptide chains, but also on the detailed folding of the chains themselves and on their assembly into larger supramolecular structures. The molecules and their fu- tional assemblies possess a limited in vitro stability. Special methods are required for their intact isolation from the source material and for their analysis, both qualitatively and quantitatively. Proteins are also increasingly used as “industrial components,” e.g., in biosensors and immobilized enzymes, because of their specificity, selectivity, and sensitivity. This requires novel and refined proce- ing methods by which the protein isolate can be converted into a form in which it can be utilized.

Keywords

amino acid biosensor enzymes membrane receptor protein protein engineering proteins receptor

Editors and affiliations

  • Felix Franks
    • 1
  1. 1.Pafra Ltd.CambridgeUK

Bibliographic information