Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1531)
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Table of contents (19 chapters)
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Back Matter
Keywords
About this book
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
Editors and Affiliations
Bibliographic Information
Book Title: Type 3 Secretion Systems
Book Subtitle: Methods and Protocols
Editors: Matthew L. Nilles, Danielle L. Jessen Condry
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6649-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6647-9Published: 12 November 2016
Softcover ISBN: 978-1-4939-8260-8Published: 27 June 2018
eBook ISBN: 978-1-4939-6649-3Published: 11 November 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 224
Number of Illustrations: 9 b/w illustrations, 23 illustrations in colour
Topics: Microbiology