Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1377)
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About this book
This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.
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Keywords
Table of contents (43 protocols)
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Protein Production, Isolation, Purification, and Stabilization
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Activity Assays
Editors and Affiliations
Bibliographic Information
Book Title: P-Type ATPases
Book Subtitle: Methods and Protocols
Editors: Maike Bublitz
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3179-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3178-1Published: 23 December 2015
Softcover ISBN: 978-1-4939-7995-0Published: 29 March 2019
eBook ISBN: 978-1-4939-3179-8Published: 22 December 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 544
Number of Illustrations: 56 b/w illustrations, 51 illustrations in colour
Topics: Membrane Biology