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Mammalian Synthetic Systems

  • Book
  • © 2024

Overview

Editors:
  1. Francesca Ceroni

    1. Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Karen Polizzi

    1. Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2774)

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About this book

This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Mammalian Synthetic Systems aims to ensure successful results in the further study of this vital field.

Keywords

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. A Directed Evolution Protocol for Engineering Minimal Transcription Factors, Based on CIS Display

    • Lin Qi, Emily Bennett, Mark Isalan
    Pages 1-13

  3. Setup and Applications of Modular Protein Expression Toolboxes (MoPET) for Mammalian Systems

    • Ernst Weber
    Pages 15-29

  4. Coiled-Coil Interaction Toolbox for Engineering Mammalian Cells

    • Erik Rihtar, Tina Fink, Roman Jerala
    Pages 31-41

  5. A Mammalian-Based Synthetic Biology Toolbox to Engineer Membrane–Membrane Interfaces

    • Hossein Moghimianavval, Sonisilpa Mohapatra, Allen P. Liu
    Pages 43-58

  6. Engineered Protease-Responsive RNA-Binding Proteins (RBPs) to Expand the Toolbox of Synthetic Circuits in Mammalian Cells

    • Fabiana Calandra, Velia Siciliano
    Pages 59-69

  7. Mechanistic Model-Driven Biodesign in Mammalian Synthetic Biology

    • Yin Hoon Chew, Lucia Marucci
    Pages 71-84

  8. Realizing Antithetic Integral Feedback Control in Mammalian Cells

    • Timothy Frei, Mustafa Khammash
    Pages 85-98

  9. A Computational Modeling Approach for the Design of Genetic Control Systems that Respond to Transcriptional Activity

    • Carlos D. Llanos, Tianyi Xie, Ha Eun Lim, Laura Segatori
    Pages 99-117

  10. Flux Balance Analysis of Mammalian Cell Systems

    • James Morrissey, Benjamin Strain, Cleo Kontoravdi
    Pages 119-134

  11. Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Copy Genomic Integration and High-Throughput DNA Sequencing

    • Nisha D. Kamath, Kenneth A. Matreyek
    Pages 135-152

  12. Flow Cytometry Quantification of Transient Transfections in Mammalian Cells

    • Jacob Beal
    Pages 153-176

  13. RNA Switches Using Cas Proteins

    • Moe Hirosawa, Hirohide Saito
    Pages 177-192

  14. Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR

    • James W. Bryson, Susan J. Rosser
    Pages 193-204

  15. Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems

    • Carolin Maja Gebhardt, Dominik Niopek
    Pages 205-231

  16. Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells

    • Maysam Mansouri, Martin Fussenegger
    Pages 233-241

  17. Detection of MicroRNAs Using Synthetic Toehold Switch in Mammalian Cells

    • Yuwen Zhao, Pratima Poudel, Shue Wang
    Pages 243-258

  18. Imaging S-Adenosyl Methionine Dynamics in Living Cells Using an RNA-Based Fluorescent Sensor

    • Jared D. Moon, Kevin Yusko, Lindsey Nassimos, Jiahui Wu
    Pages 259-267

  19. High-Throughput Spectroscopic Analysis of mRNA Capping Level

    • Chileab Redwood-Sawyerr, Rochelle Aw, Roberto Di Blasi, Ignacio Moya-Ramírez, Cleo Kontoravdi, Francesca Ceroni et al.
    Pages 269-278

  20. In Vitro Generation of Megakaryocytes from Engineered Mouse Embryonic Stem Cells

    • Mitchell R. Lewis, Tara L. Deans
    Pages 279-301
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Editors and Affiliations

  • Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    Francesca Ceroni, Karen Polizzi

Bibliographic Information

  • Book Title: Mammalian Synthetic Systems

  • Editors: Francesca Ceroni, Karen Polizzi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3718-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3717-3Published: 06 March 2024

  • Softcover ISBN: 978-1-0716-3720-3Due: 15 April 2024

  • eBook ISBN: 978-1-0716-3718-0Published: 05 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 316

  • Number of Illustrations: 88 b/w illustrations

  • Topics: Biotechnology, Biological Techniques

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