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Bacterial Secretion Systems

Methods and Protocols

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  • © 2024
  • Latest edition

Overview

Editors:
  1. Laure Journet

    1. Laboratoire d’Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ, CNRS, Marseille, France

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  2. Eric Cascales

    1. Laboratoire d’Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ, CNRS, Marseille, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2715)

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About this book

This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful andpractical guide to new researchers and experts looking to expand their knowledge. 

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder Version 2

    • Sophie S. Abby, Rémi Denise, Eduardo P. C. Rocha
    Pages 1-25

  3. Protein Sorting Prediction

    • Henrik Nielsen
    Pages 27-63

  4. Cell Fractionation

    • Melissa Petiti, Laetitia Houot, Denis Duché
    Pages 65-71

  5. Components Subcellular Localization: Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

    • Nienke Buddelmeijer
    Pages 73-78

  6. Components Subcellular Localization: Identification of Lipoproteins Using Alkyne Fatty Acids and Click Chemistry

    • Karine Nozeret, Nienke Buddelmeijer
    Pages 79-89

  7. Defining Membrane Protein Localization by Isopycnic Density Gradients

    • Rhys A. Dunstan, Iain D. Hay, Trevor Lithgow
    Pages 91-98

  8. Components Subcellular Localization: Cell Surface Exposure

    • Anna Konovalova
    Pages 99-110

  9. Probing Protein Topology and Conformation by Limited Proteolysis

    • Maïalène Chabalier, Thierry Doan, Eric Cascales
    Pages 111-119

  10. Exploring Uniform, Dual, and Dynamic Topologies of Membrane Proteins by Substituted Cysteine Accessibility Method (SCAM™)

    • Mikhail Bogdanov
    Pages 121-157

  11. Preparation of Uniformly Oriented Inverted Inner (Cytoplasmic) Membrane Vesicles from Gram-Negative Bacterial Cells

    • Mikhail Bogdanov
    Pages 159-180

  12. Defining Membrane Protein Topology Using pho-lac Reporter Fusions

    • Gouzel Karimova, Daniel Ladant
    Pages 181-195

  13. Measure of Peptidoglycan Degradation Activity

    • Yoann G. Santin, Eric Cascales
    Pages 197-205

  14. Protein–Protein Interaction: Bacterial Two Hybrid

    • Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, Daniel Ladant
    Pages 207-224

  15. Protein–Protein Interactions: Oxidative Bacterial Two Hybrid

    • Callypso Pellegri, Emmanuelle Bouveret, Laetitia Houot
    Pages 225-233

  16. Protein–Protein Interactions: Yeast Two Hybrid

    • Jer-Sheng Lin, Erh-Min Lai
    Pages 235-246

  17. Protein–Protein Interactions: Bimolecular Fluorescence Complementation and Cytology Two Hybrid

    • Dyuti Purkait, Mohd Ilyas, Krishnamohan Atmakuri
    Pages 247-257

  18. Bacterial One- and Two-Hybrid Assays to Monitor Transmembrane Helix Interactions

    • Abdelrahim Zoued, Jean-Pierre Duneau, Eric Cascales
    Pages 259-271

  19. Protein–Protein Interactions: Co-immunoprecipitation

    • Jer-Sheng Lin, Jemal Ali, Erh-Min Lai
    Pages 273-283

  20. Protein–Protein Interaction: Tandem Affinity Purification in Bacteria

    • Julie P. M. Viala, Emmanuelle Bouveret
    Pages 285-297
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Editors and Affiliations

  • Laboratoire d’Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ, CNRS, Marseille, France

    Laure Journet, Eric Cascales

Bibliographic Information

  • Book Title: Bacterial Secretion Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Laure Journet, Eric Cascales

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3445-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3444-8Published: 07 November 2023

  • Softcover ISBN: 978-1-0716-3447-9Due: 08 December 2023

  • eBook ISBN: 978-1-0716-3445-5Published: 06 November 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 613

  • Number of Illustrations: 23 b/w illustrations, 97 illustrations in colour

  • Topics: Microbiology

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