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Regulatory T-Cells

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Masahiro Ono

    1. Imperial College London, London, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2559)

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About this book

This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Regulatory T-Cells: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Immunological Techniques for Analysing Treg

    1. Front Matter

      Pages 1-1
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    2. Analysis of the In Vivo Function of Follicular Regulatory T (TFR) Cells in the Regulation of Antibody Response

      • Lin Luo, Jianmei W. Leavenworth
      Pages 3-13

    3. Adoptive Transfer and Bone Marrow Chimera Models to Analyze Treg Function and Differentiation

      • Vital da Silva Domingues, Iris Caramalho, Marie-Louise Bergman, Jocelyne Demengeot
      Pages 15-29

    4. Depletion of Treg by the Diphtheria Toxin System

      • Sho Hanakawa, Zachary Chow, Kenji Kabashima
      Pages 31-39

    5. Analysis of Peripherally Derived Treg in the Intestine

      • Tadashi Takeuchi, Hiroshi Ohno
      Pages 41-49

    6. Isolation and Analysis of Tumor-Infiltrating Treg

      • Katharina Bergerhoff, Malin Pedersen
      Pages 51-63

  3. Molecular Techniques for Analysing Treg

    1. Front Matter

      Pages 65-65
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    2. Differentiation of Peripheral Treg

      • José Almeida-Santos, Marie-Louise Bergman, Jocelyne Demengeot
      Pages 67-77

    3. Functional Analysis of Foxp3 and Its Mutants by Retroviral Transduction of Murine Primary CD4+ T Cells

      • Akira Nakajima, Ryuichi Murakami, Shohei Hori
      Pages 79-94

    4. Genetic Tools for Analyzing Foxp3+ Treg Cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping

      • Mario Simonetti, Acelya Yilmazer, Karsten Kretschmer
      Pages 95-114

    5. Analysis of FOXP3 DNA Methylation Patterns to Identify Functional FOXP3+ T-Cell Subpopulations

      • Ekaterina Minskaia, João F. Lacerda
      Pages 115-136

    6. Protein Ubiquitination Assay

      • Ping Wei, Rui Liang, Fan Pan
      Pages 137-149

  4. Human Treg Analysis and Clinical Application

    1. Front Matter

      Pages 151-151
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    2. Phenotypic and Functional Studies of Human Treg Cell Subpopulations

      • Mo Atif, Mustapha Cherai, Makoto Miyara
      Pages 153-169

    3. In Vitro Analysis of CTLA-4-Mediated Transendocytosis by Regulatory T Cells

      • Erin Waters, Cayman Williams, Alan Kennedy, David M. Sansom
      Pages 171-187

    4. Isolation and Functional Characterization of Regulatory CD4+ T Cells from the Inflamed Joints of Patients with Rheumatoid Arthritis

      • Giovanni A. M. Povoleri, Veerle Fleskens, Leonie S. Taams
      Pages 189-203

    5. A GMP Protocol for the Manufacture of Tregs for Clinical Application

      • Alice O’Neil, Matthew Brook, Seetha Abdul-Wahab, Joanna Hester, Giovanna Lombardi, Fadi Issa
      Pages 205-227

  5. Single Cell and High-Dimensional Techniques for Analysing Treg

    1. Front Matter

      Pages 229-229
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    2. Regulatory T-Cell Phenotyping Using CyTOF

      • Hugo Barcenilla, Mikael Pihl, Florence Sjögren, Louise Magnusson, Rosaura Casas
      Pages 231-242
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Editors and Affiliations

  • Imperial College London, London, UK

    Masahiro Ono

Bibliographic Information

  • Book Title: Regulatory T-Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Masahiro Ono

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2647-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2646-7Published: 01 October 2022

  • Softcover ISBN: 978-1-0716-2649-8Published: 02 October 2023

  • eBook ISBN: 978-1-0716-2647-4Published: 30 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 280

  • Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour

  • Topics: Immunology

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