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Effector-Triggered Immunity

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Thomas A. Kufer

    1. Department of Immunology, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Maria Kaparakis-Liaskos

    1. Department of Physiology Anatomy & Microbiology, La Trobe University, Bundoora, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detailed essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2523)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. A Brief Introduction to Effector-Triggered Immunity

    • Thomas A. Kufer, Maria Kaparakis-Liaskos
    Pages 1-8

  3. Lambda Red Recombineering in Shigella flexneri

    • Adrian Herod, Julie Ryu, John Rohde
    Pages 9-21

  4. Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity During Legionella pneumophila Infection

    • Rachelia R. Wibawa, Pengfei Li, Kathleen McCaffrey, Elizabeth L. Hartland
    Pages 23-41

  5. Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of Their Immunogenic Functions

    • Natalie J. Bitto, Maria Kaparakis-Liaskos
    Pages 43-61

  6. Gene Gun-Mediated Transient Gene Expression for Functional Studies in Plant Immunity

    • Franz Leissing, Anja Reinstädler, Hannah Thieron, Ralph Panstruga
    Pages 63-77

  7. Host-Induced Gene Silencing Using BPMV on Soybean to Study Genes in the Soybean Rust Fungus Phakopsora pachyrhizi

    • Tobias I. Link
    Pages 79-91

  8. Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors

    • Kostya Kanyuka
    Pages 93-112

  9. Time-Resolved Fluorescence Microscopy Screens on Host Protein Subversion During Bacterial Cell Invasion

    • Lisa Sanchez, Yuen-Yan Chang, Nora Mellouk, Jost Enninga
    Pages 113-131

  10. Assaying RIPK2 Activation by Complex Formation

    • Heidrun Steinle, Kornelia Ellwanger, Thomas A. Kufer
    Pages 133-150

  11. Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1 or NOD2 with Sphingosine-1-Phosphate Using Microscale Thermophoresis

    • Gang Pei
    Pages 151-160

  12. A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Associated Factors

    • Jonas Moecking, Matthias Geyer
    Pages 161-177

  13. Functional Assessment of Disease-Associated Pyrin Variants

    • Daria Chirita, Yvan Jamilloux, Thomas Henry, Flora Magnotti
    Pages 179-195

  14. An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions

    • Inga V. Hochheiser, Matthias Geyer
    Pages 197-207

  15. Detection of Gasdermin Activation and Lytic Cell Death During Pyroptosis and Apoptosis

    • Benjamin Demarco, Saray Ramos, Petr Broz
    Pages 209-237

  16. Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging

    • Seong H. Chow, Thomas Naderer
    Pages 239-252

  17. Non-apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation

    • Akshay A. D’Cruz, Maria Ericsson, Ben A. Croker
    Pages 253-263

  18. Analysis of Bacteria-Triggered Inflammasome: Activation in Neutrophils by Immunoblot

    • Rémi Planès, Karin Santoni, Etienne Meunier
    Pages 265-279

  19. Quantifying Regulated Mitochondrial Fission in Macrophages

    • Syeda Farhana Afroz, Nicholas D. Condon, Matthew J. Sweet, Ronan Kapetanovic
    Pages 281-301

  20. Live Cell Imaging of T Cell Pyroptosis

    • Andreas Linder, Veit Hornung
    Pages 303-315
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Keywords

About this book

This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key host responses; and techniques used for the analysis of inflammasome activation, cell death pathways, and mitochondria damage in response to pathogens. All of these topics cover a broad spectrum of immunological, biochemical, cell biological, and structural biology approaches to examine ETI. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Effector-Triggered Immunity: Methods and Protocols is a valuable resource for both expert and novice researchers who are interested in learning more about the important and developing field of ETI.


Editors and Affiliations

  • Department of Immunology, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany

    Thomas A. Kufer

  • Department of Physiology Anatomy & Microbiology, La Trobe University, Bundoora, Australia

    Maria Kaparakis-Liaskos

Bibliographic Information

  • Book Title: Effector-Triggered Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas A. Kufer, Maria Kaparakis-Liaskos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2449-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2448-7Published: 28 June 2022

  • Softcover ISBN: 978-1-0716-2451-7Published: 28 June 2022

  • eBook ISBN: 978-1-0716-2449-4Published: 27 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 319

  • Number of Illustrations: 8 b/w illustrations, 41 illustrations in colour

  • Topics: Immunology, Protein Science, Pathology

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