Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible result
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2392)
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About this book
This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
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Keywords
Table of contents (18 protocols)
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Primer Design for Identification of Plant and Animal Viruses
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Use of Software for Primer Design
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Primer Design for Newer PCR Approaches
Editors and Affiliations
Bibliographic Information
Book Title: PCR Primer Design
Editors: Chhandak Basu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1799-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1798-4Published: 14 November 2021
Softcover ISBN: 978-1-0716-1801-1Published: 14 November 2022
eBook ISBN: 978-1-0716-1799-1Published: 13 November 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIII, 276
Number of Illustrations: 90 b/w illustrations
Topics: Human Genetics