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Mammalian Cell Engineering

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ryosuke Kojima

    1. Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts.

Part of the book series: Methods in Molecular Biology (MIMB, volume 2312)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Engineering Mammalian Cells to Sense Biologically Relevant Inputs

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Generation of CAR-T Cells by Lentiviral Transduction

      • Atsushi Okuma
      Pages 3-14

    3. Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells

      • Leo Scheller
      Pages 15-33

    4. Engineering Mammalian Cells to Control Glucose Homeostasis

      • Jiawei Shao, Xinyuan Qiu, Mingqi Xie
      Pages 35-57

    5. Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System

      • Akihiro Eguchi, Masahiro Kawahara
      Pages 59-72

    6. Purification of Specific Cell Populations Differentiated from Stem Cells Using MicroRNA-Responsive Synthetic Messenger RNAs

      • Hideyuki Nakanishi, Hirohide Saito
      Pages 73-86

  3. Engineering Mammalian Cells to Sense Artificial Inputs

    1. Front Matter

      Pages 87-87
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    2. Green Light-Controlled Gene Switch for Mammalian and Plant Cells

      • Nils Schneider, Claire V. Chatelle, Rocio Ochoa-Fernandez, Matias D. Zurbriggen, Wilfried Weber
      Pages 89-107

    3. Sonogenetic Modulation of Cellular Activities in Mammalian Cells

      • Yao-Shen Huang, Ching-Hsiang Fan, Wei-Ting Yang, Chih-Kuang Yeh, Yu-Chun Lin
      Pages 109-124

    4. Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells

      • Yuanhuan Yu, Guiling Yu, Haifeng Ye
      Pages 125-139

    5. Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice

      • Guiling Yu, Yuanhuan Yu, Haifeng Ye
      Pages 141-158

    6. Construction of Caffeine-Inducible Gene Switches in Mammalian Cells

      • Daniel Bojar
      Pages 159-168

  4. Precise Genome Engineering Techniques Using CRISPR-Cas Systems

    1. Front Matter

      Pages 169-169
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    2. Multiplexed Genome Engineering with Cas12a

      • Niels R. Weisbach, Ab Meijs, Randall J. Platt
      Pages 171-192

    3. Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells

      • Soh Ishiguro, Nozomu Yachie
      Pages 193-223

    4. Optical Control of Genome Editing by Photoactivatable Cas9

      • Takahiro Otabe, Yuta Nihongaki, Moritoshi Sato
      Pages 225-233

  5. Engineering Mammalian Cells in Combination with Chemical Compounds/Systems

    1. Front Matter

      Pages 235-235
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    2. Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT

      • Sachio Suzuki, Yuka Hatano, Tatsuyuki Yoshii, Shinya Tsukiji
      Pages 237-251

    3. Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules

      • Hideki Nakamura
      Pages 253-276
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Keywords

About this book

This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.


Editors and Affiliations

  • Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Ryosuke Kojima

Bibliographic Information

  • Book Title: Mammalian Cell Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Ryosuke Kojima

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1441-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1440-2Published: 07 July 2021

  • Softcover ISBN: 978-1-0716-1443-3Published: 07 July 2022

  • eBook ISBN: 978-1-0716-1441-9Published: 06 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 330

  • Number of Illustrations: 16 b/w illustrations, 67 illustrations in colour

  • Topics: Biomedical Engineering/Biotechnology

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