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In Vitro Models for Stem Cell Therapy

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Peggy Stock

    1. Department of Visceral, Transplant, Thoracic and Vascular Surgery, Applied Molecular Hepatology Lab, University of Leipzig Medical Center, Leipzig, Germany

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  2. Bruno Christ

    1. Department of Visceral, Transplant, Thoracic and Vascular Surgery, Applied Molecular Hepatology Lab, University of Leipzig Medical Center, Leipzig, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2269)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Consistent Inclusion of Mesenchymal Stem Cells into In Vitro Tumor Models

      • Luís P. Ferreira, Vítor M. Gaspar, João F. Mano
      Pages 3-23

    3. Pluripotent Stem Cells for Cell Therapy

      • Insa S. Schroeder
      Pages 25-33

  3. Disease Modeling

    1. Front Matter

      Pages 35-35
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    2. In Vitro Methods for the Study of Glioblastoma Stem-Like Cell Radiosensitivity

      • Joseph H. McAbee, Charlotte Degorre-Kerbaul, Philip J. Tofilon
      Pages 37-47

    3. Bioimaging of Mesenchymal Stem Cells Spatial Distribution and Interactions with 3D In Vitro Tumor Spheroids

      • Luís P. Ferreira, Vítor M. Gaspar, João F. Mano
      Pages 49-61

    4. Investigation of the MSC Paracrine Effects on Alveolar–Capillary Barrier Integrity in the In Vitro Models of ARDS

      • Johnatas Dutra Silva, Anna D. Krasnodembskaya
      Pages 63-81

    5. In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on TGF-β1-Induced Pulmonary Fibrosis

      • Ying-Wei Lan, Chuan-Mu Chen, Kowit-Yu Chong
      Pages 83-92

    6. Study of Mesenchymal Stem Cell-Mediated Mitochondrial Transfer in In Vitro Models of Oxidant-Mediated Airway Epithelial and Smooth Muscle Cell Injury

      • Charalambos Michaeloudes, Xiang Li, Judith C. W. Mak, Pankaj K. Bhavsar
      Pages 93-105

    7. Co-Culture of Peripheral Blood Mononuclear Cells and Endothelial Colony Forming Cells from Cord Blood of Preterm Born Babies

      • Jan Baier, Anchang Charles Gwellem, Roland Haase, Ines Volkmer, Babette Bartling, Martin S. Staege
      Pages 107-124

    8. Ex Vivo Model of Spontaneous Neuroretinal Degeneration for Evaluating Stem Cells’ Paracrine Properties

      • Ivan Fernandez-Bueno, Ricardo Usategui-Martin
      Pages 125-137

    9. Ex Vivo Normothermic Hypoxic Rat Liver Perfusion Model: An Experimental Setting for Organ Recondition and Pharmacological Intervention

      • Federica Rigo, Victor Navarro-Tableros, Nicola De Stefano, Alberto Calleri, Renato Romagnoli
      Pages 139-150

    10. Co-Culture of Human Mesenchymal Stromal Cells and Primary Mouse Hepatocytes

      • Mei-Ju Hsu, Madlen Christ, Bruno Christ
      Pages 151-165

    11. A 3D Dynamic In Vitro Model of Inflammatory Tendon Disease

      • Susanna Schubert, Luisa Brandt, Janina Burk
      Pages 167-174

    12. Generation of Epidermal Equivalents from Hair Follicle Melanocytes, Keratinocytes, and Dermal Fibroblasts

      • Vuk Savkovic, Marie Schneider, Hanluo Li, Jan-Christoph Simon, Mirjana Ziemer, Bernd Lethaus
      Pages 175-201

  4. Characterization and Pre-Conditioning

    1. Front Matter

      Pages 203-203
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    2. Using Gene Expression Music Algorithms (GEMusicA) for the Characterization of Human Stem Cells

      • Martin S. Staege
      Pages 205-219

    3. Evaluation of Extracellular Vesicles from Adipose Tissue-Derived Mesenchymal Stem Cells in Primary Human Chondrocytes from Patients with Osteoarthritis

      • María Isabel Guillén, Alvaro Compañ, María José Alcaraz
      Pages 221-231

    4. Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development

      • Matthias Jung, Jovita Schiller, Carla Hartmann, Jenny Pfeifer, Dan Rujescu
      Pages 233-244
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Keywords

About this book

This volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field



Editors and Affiliations

  • Department of Visceral, Transplant, Thoracic and Vascular Surgery, Applied Molecular Hepatology Lab, University of Leipzig Medical Center, Leipzig, Germany

    Peggy Stock, Bruno Christ

Bibliographic Information

  • Book Title: In Vitro Models for Stem Cell Therapy

  • Book Subtitle: Methods and Protocols

  • Editors: Peggy Stock, Bruno Christ

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1225-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1224-8Published: 10 March 2021

  • Softcover ISBN: 978-1-0716-1227-9Published: 10 March 2022

  • eBook ISBN: 978-1-0716-1225-5Published: 09 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 284

  • Number of Illustrations: 13 b/w illustrations, 42 illustrations in colour

  • Topics: Stem Cells, Immunology

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