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Stem Cells in Regenerative Medicine

  • Book
  • © 2009

Overview

Editors:
  1. Julie Audet

    1. University of Toronto, Toronto, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. William L. Stanford

    1. University of Toronto, Toronto, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides both in vivo and in vitro assays
  • Includes protocols for both adult and embryonic stem cells

Part of the book series: Methods in Molecular Biology (MIMB, volume 482)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xix
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  2. Stem Cell Sources and Manipulation

    1. Front Matter

      Pages 1-1
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    2. Derivation and Manipulation of Murine Embryonic Stem Cells

      • Alexander Meissner, Sarah Eminli, Rudolf Jaenisch
      Pages 3-19

    3. Patterning Mouse and Human Embryonic Stem Cells Using Micro-contact Printing

      • Raheem Peerani, Celine Bauwens, Eugenia Kumacheva, Peter W. Zandstra
      Pages 21-33

    4. Efficient Gene Knockdowns in Human Embryonic Stem Cells Using Lentiviral-Based RNAi

      • Asmin Tulpule, George Q. Daley
      Pages 35-42

    5. Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells

      • Aziza P. Manceur, Julie Audet
      Pages 43-54

    6. Stem Cell Sources for Regenerative Medicine

      • Ali M. Riazi, Sarah Y. Kwon, William L. Stanford
      Pages 55-90

  4. Regeneration of the Nervous System

    1. Front Matter

      Pages 141-141
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    2. Isolation and Manipulation of Mammalian Neural Stem Cells In Vitro

      • Claudio Giachino, Onur Basak, Verdon Taylor
      Pages 143-158

    3. Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors

      • Karl J.L. Fernandes, Freda D. Miller
      Pages 159-170

    4. Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Cord

      • Hynek Wichterle, Mirza Peljto, Stephane Nedelec
      Pages 171-183

    5. Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS

      • Tong Zheng, Gregory P. Marshall II II, K. Amy Chen, Eric D. Laywell
      Pages 185-197

    6. Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys

      • Jun Takahashi, Yasushi Takagi, Hidemoto Saiki
      Pages 199-212

  5. Regeneration of the Epidermis

    1. Front Matter

      Pages 213-213
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    2. Isolation and Culture of Epithelial Stem Cells

      • Jonathan A. Nowak, Elaine Fuchs
      Pages 215-232

    3. Regeneration of Skin and Cornea by Tissue Engineering

      • Danielle Larouche, Claudie Paquet, Julie Fradette, Patrick Carrier, François A. Auger, Lucie Germain
      Pages 233-256
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Keywords

About this book

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology™ series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.

Editors and Affiliations

  • University of Toronto, Toronto, Canada

    Julie Audet, William L. Stanford

Bibliographic Information

  • Book Title: Stem Cells in Regenerative Medicine

  • Editors: Julie Audet, William L. Stanford

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-060-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-797-6Published: 12 December 2008

  • Softcover ISBN: 978-1-61737-784-6Published: 19 January 2011

  • eBook ISBN: 978-1-59745-060-7Published: 17 December 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 424

  • Number of Illustrations: 75 b/w illustrations

  • Topics: Cell Biology, Stem Cells

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