Abstract
Neural transplantation has been a long-standing goal for the treatment of neurological injury and disease. The recent discovery of persistent pools of neural stem cells within the adult mammalian brain has reignited interest in transplant therapeutics. Since neural stem cells are self-renewing, it may be possible to culture and expand neural stem cells and their progenitor cell progeny to sufficient numbers for use in autologous, self-repair strategies. Such approaches will require optimized cultivation protocols, as well as extensive testing of candidate donor cells to assess their capacity for engraftment, survival, and integration. In this chapter, we describe the transplantation of neural stem/progenitor cells—cultivated as either neurospheres or neurogenic astrocyte monolayers—into the persistently neurogenic olfactory bulb system of the adult mouse forebrain, and into the cerebellum of neonatal mutant mice.
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References
Bjorklund, A., Stenevi, U. (1979) Reconstruction of the nigrostriatal dopamine pathway by intracerebral nigral transplants. Brain Res. 177:555–60.
Madrazo, I., Drucker-Colin, R., Diaz, V., Martinez-Mata J., Torres, C., Becerri, J.J. (1987) Open microsurgical autograft of adrenal medulla to the right caudate nucleus in two patients with intractable Parkinson's disease. N Engl J Med. 316:831–4.
Fernandez-Espejo, E., Armengol, J.A., Flores, J.A., Galan-Rodriguez, B., Ramiro, S. (2005) Cells of the sympathoadrenal lineage: biological properties as donor tissue for cell-replacement therapies for Parkinson's disease. Brain Res Brain Res Rev. 49:343–54.
Winkler, C., Kirik, D., Bjorklund, A. (2005) Cell transplantation in Parkinson’s disease: how can we make it work? Trends Neurosci. 28:86–92.
Correia, A.S., Anisimov, S.V., Li, J-Y., Brundin, P. (2005) Stem cell-based therapy for Parkinson’s disease. Ann Med. 37:487–98.
Freed, W.J., Cannon-Spoor, H.E., Krauthammer, E. (1987) Intrastriatal adrenal medulla grafts in rats. Long-term survival and behavioral effects. J Neurosurg. 65:664–70.
Reynolds, B.A., Weiss, S. (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255:1707–10.
Reynolds, B.A., Rietze, R.L. (2005) Neural stem cells and neurospheres—re-evaluating the relationship. Nat Meth. 2:333–6.
Laywell, E.D., Rakic, P., Kukekov, V.G., Holland, E.C., Steindler, D.A. (2000) Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain. Proc Natl Acad Sci USA. 97:13883–8.
Scheffler, B., Walton, N.M., Lin, D.D., Goetz, A.K., Enikolopov, G., Roper, S.N., Steindler, D.A. (2005). Phenotypic and functional characterization of adult brain neuropoiesis. Proc Natl Acad Sci USA. 102:9353–8.
Zheng, T., Steindler, D.A., Laywell, E.D. (2002) Transplantation of an indigenous neural stem cell population leading to hyperplasia and atypical integration. Clon Stem Cells 4:3–8.
Zheng, T., Marshall, G.P., II, Laywell, E.D., Steindler, D.A. (2006a). Neurogenic astrocytes transplanted into the adult mouse lateral ventricle contribute to olfactory neurogenesis, and reveal a novel intrinsic subependymal neuron. Neurosci. 142:175–85.
Deng, J., Steindler, D.A., Petersen, B.E., Laywell, E.D. (2003). Neural trans-differentiation potential of hepatic oval cells in the neonatal mouse brain. Exp Neurol. 182:373–382.
Marshall, G.P., II, Scott, E.W., Zheng, T., Laywell, E.D., Steindler, D.A. (2005). Ionizing irradiation enhances the engraftment of transplanted in vitro-derived neural multipotent astrocytic stem cells. Stem Cells 23:1276–85.
Zheng, T., Rossignol, C., Leibovici, A., Anderson, K.J., Steindler, D.A., Weiss, M.D. (2006) Transplantation of multipotent astrocytic stem cells into a rat model of neonatal hypoxic-ischemic encephalopathy. Brain Res. 1112:99–105.
Laywell, E.D., Kukekov, V.G., Suslov, O., Zheng, T., Steindler, D.A. (2002) Production and analysis of neurospheres from acutely dissociated and postmortem CNS specimens. Meth Mol Biol. 198:15–27.
Seaberg, R.M., van der Kooy, D. (2003) Stem and progenitor cells: the premature desertion of rigorous definitions. Trends Neurosci. 26:125–31.
Phifer, C.B., Terry, L.M. (1986). Use of hypothermia for general anesthesia in preweanling rodents. Physiol Behav. 38:887–90.
Walton, N.M., Sutter, B.M., Laywell, E.D., Levkoff, L.H., Kearns, S.M., Marshall, G.P., II, Scheffler, B., Steindler, D.A. (2006). Microglia instruct subventricular zone neurogenesis. GLIA 54:815–25.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Zheng, T., Marshall II, G.P., Chen, K.A., Laywell, E.D. (2009). Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS. In: Audet, J., Stanford, W.L. (eds) Stem Cells in Regenerative Medicine. Methods in Molecular Biology, vol 482. Humana Press. https://doi.org/10.1007/978-1-59745-060-7_12
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DOI: https://doi.org/10.1007/978-1-59745-060-7_12
Publisher Name: Humana Press
Print ISBN: 978-1-58829-797-6
Online ISBN: 978-1-59745-060-7
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