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Hair Growth-Promoting Effects of Lavender Oil in C57BL/6 Mice

Abstract

The purpose of this study was to determine the hair growth effects of lavender oil (LO) in female C57BL/6 mice. The experimental animals were divided into a normal group (N: saline), a vehicle control group (VC: jojoba oil), a positive control group (PC: 3% minoxidil), experimental group 1 (E1: 3% LO), and experimental group 2 (E2: 5% LO). Test compound solutions were topically applied to the backs of the mice (100 µL per application), once per day, 5 times a week, for 4 weeks. The changes in hair follicle number, dermal thickness, and hair follicle depth were observed in skin tissues stained with hematoxylin and eosin, and the number of mast cells was measured in the dermal and hypodermal layers stained with toluidine blue. PC, E1, and E2 groups showed a significantly increased number of hair follicles, deepened hair follicle depth, and thickened dermal layer, along with a significantly decreased number of mast cells compared to the N group. These results indicated that LO has a marked hair growth-promoting effect, as observed morphologically and histologically. There was no significant difference in the weight of the thymus among the groups. However, both absolute and relative weights of the spleen were significantly higher in the PC group than in the N, VC, E1, or E2 group at week 4. Thus, LO could be practically applied as a hair growth-promoting agent.

References

  1. Hadshiew, I.M., Foitzik, K., Arck, P.C. and Paus, R. (2004) Burden of hair loss: stress and the underestimated psychosocial impact of telogen effluvium and androgenetic alopecia. J. Invest. Dermatol., 123, 455–457.

    CAS  Article  Google Scholar 

  2. Rho, S.S., Park, S.J., Hwang, S.L., Lee, M.H., Kim, C.D., Lee, I.H., Chang, S.Y. and Rang, M.J. (2005) The hair growth promoting effect of Asiasari radix extract and its molecular regulation. J. Dermatol. Sci., 38, 89–97.

    Article  Google Scholar 

  3. Cash, T.F., Price, V.H. and Savin, R.C. (1993) Psychological effects of androgenetic alopecia on women: comparisons with balding men and with female control subjects. J. Am. Acad. Dematol., 29, 568–575.

    CAS  Article  Google Scholar 

  4. Alonso, L. and Fuchs, E. (2006) The hair cycle. J. Cell Sci., 119, 391–393.

    CAS  Article  Google Scholar 

  5. Paus, R., Christoph, T. and Müller-Röver, S. (1999) Immunology of the hair follicle: a short journey into terra incognita. J. Investig. Dermatol. Symp. Proc., 4, 226–234.

    CAS  Article  Google Scholar 

  6. Van Neste, D. and Tobin, D.J. (2004) Hair cycle and hair pigmentation: dynamic interactions and changes associated with aging. Micron, 35, 193–200.

    Article  Google Scholar 

  7. Messenger, A.G. and Rundegren, J. (2004) Minoxidil: mechanisms of action on hair growth. Br. J. Dermatol., 150, 186–194.

    CAS  Article  Google Scholar 

  8. Guin, J.D. (1981) Complications of topical hydrocortisone. J. Am. Acad. Dermatol., 4, 417–422.

    CAS  Article  Google Scholar 

  9. Bouseta, A., Scheirman, V. and Collin, S. (1996) Flavor and free amino acid composition of lavender and eucalyptus honeys. J. Food Sci., 61, 683–687.

    CAS  Article  Google Scholar 

  10. Slominski, A. and Paus, R. (1993) Melanogenesis is coupled to murine anagen: toward new concepts for the role of melanocytes and the regulation of melanogenesis in hair growth. J. Invest. Dermatol., 101, 90S–97S.

    CAS  Article  Google Scholar 

  11. Müller-Röver, S., Handjiski, B., van der Veen, C., Eichmüller, S., Foitzik, K., Mckay, I.A., Stenn, K.S. and Paus, R. (2001) A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J. Invest. Dermatol., 117, 3–15.

    Article  Google Scholar 

  12. Sun, L.Q., Lee, D.W., Zhang, Q., Xiao, W., Raabe, E.H., Meeker, A., Miao, D., Huso, D.L. and Arceci, R.J. (2004) Growth retardation and premature aging phenotypes in mice with disruption of the SNF2-like gene, PASG. Genes Dev., 18, 1035–1046.

    CAS  Article  Google Scholar 

  13. Paus, R. and Link, R.E. (1988) The psoriatic epidermal lesion and angen hair growth may share the same “switch-on” mechanism. Yale J. Biol. Med., 61, 467–476.

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Paus, R., Maurer, M., Slominski, A. and Czametzki, B.M. (1994) Mast cell involvement in murine hair growth. Dev. Biol., 163, 230–240.

    CAS  Article  Google Scholar 

  15. Ito, N., Sugawara, K., Bodó, E., Takigawa, M., van Beek, N., Ito, T. and Paus, R. (2010) Corticotropin-releasing hormone stimulates the in situ generation of mast cells from precursors in the human hair follicle mesenchyme. J. Invest. Dermatol., 130, 995–1004.

    CAS  Article  Google Scholar 

  16. Lee, B.S. (2014) Effects of sandalwood oil on aspects of hair growth in a C57BL/6 mouse model of alopecia. J. Invest. Cosmetol., 10, 279–287.

    Article  Google Scholar 

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Correspondence to Young Chul Kim.

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Lee, B.H., Lee, J.S. & Kim, Y.C. Hair Growth-Promoting Effects of Lavender Oil in C57BL/6 Mice. Toxicol Res. 32, 103–108 (2016). https://doi.org/10.5487/TR.2016.32.2.103

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  • DOI: https://doi.org/10.5487/TR.2016.32.2.103

Key words

  • C57BL/6 mice
  • Dermal thickness
  • Hair growth
  • Hair follicle
  • Lavender oil