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Bone formation in rabbit’s leg muscle after autologous transplantation of bone marrow-derived mesenchymal stem cells expressing human bone morphogenic protein-2

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Indian Journal of Orthopaedics Aims and scope Submit manuscript

Abstract

Background

To test whether autologous transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) expressing human bone morphogenic protein-2 (hBMP-2) can produce bone in rabbit leg muscles.

Materials and Methods

MSCs were isolated from BM of the iliac crest of rabbits and then infected with lentiviral vectors (LVs) bearing hBMP-2 and green fluorescent protein under the control of the cytomegalovirus (immediate early promoter). Differentiation of transduced MSCs to osteoblasts in vitro was evaluated with an alkaline phosphatase activity assay and immuohistochemistry against osteoblast specific markers. MSCs expressing hBMP-2 were placed in an absorbable gelatin sponge, which was then transplanted into the gastrocnemius of rabbits from which MSCs were isolated. Bone formation was examined by X-ray and histological analysis.

Results

LVs efficiently mediated hBMP-2 gene expression in rabbit BM-MSCs. Ectopic expression of hBMP in these MSCs induced osteoblastic differentiation in vitro. Bone was formed after the MSCs expressing hBMP-2 were transplanted into rabbit muscles. Conclusion: Ectopic expression of hBMP-2 in rabbit MSCs induces them to differentiate into osteoblasts in vitro and to form a bone in vivo.

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Author information

Authors and Affiliations

  1. XiangYa Hospital, Central South University, 87 XiangYa Road, Changsha, Hunan, 410008, China

    Guang-Hua Lei

  2. The First Affilliated Hospital, Sun YAT-SEN University, 58 The Second ZhongShan Road, GuangZhou, GuangDong, 510080, China

    Pu-yi Sheng

  3. Department of Orthopaedics, The 8th Hospital, Changsha, No. 22, Xin Sha Road, Changsha, Hunan, 410008, China

    Licheng Wei & Han-Wen Yi

Authors
  1. Licheng Wei
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  2. Guang-Hua Lei
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  4. Pu-yi Sheng
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Correspondence to Licheng Wei.

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Wei, L., Lei, GH., Yi, HW. et al. Bone formation in rabbit’s leg muscle after autologous transplantation of bone marrow-derived mesenchymal stem cells expressing human bone morphogenic protein-2. IJOO 48, 347–353 (2014). https://doi.org/10.4103/0019-5413.136208

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  • DOI: https://doi.org/10.4103/0019-5413.136208

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