Skip to main content
SpringerLink
Log in

The effects of Oenanthe javanica extracts on hepatic fat accumulation and plasma biochemical profiles in a nonalcoholic fatty liver disease model

  • Article
  • Published:
Journal of the Korean Society for Applied Biological Chemistry Submit manuscript

Abstract

We tested the effects of Oenanthe javanica (OJ) on hepatic fat accumulation and plasma biochemical profiles in a nonalcoholic fatty liver disease model (NAFLD). Rats were divided 5 groups and fed the following diets for 6 weeks: normal rat chow diet (CHOW), high-fat highcholesterol diet (HFCD), HFCD with the water extract of OJ (HFCD+W), HFCD with the nbutanol extract of OJ (HFCD+B), and HFCD with metadoxine (HFCD+M). Metadoxine (pyridoxine-pyrrolidone-carboxylate) is being used to treat alcoholic fatty liver and has been suggested that it can be effective for treating NAFLD. The HFCD gained significantly more body weight and visceral fat mass and had significantly higher levels of plasma glucose, triglyceride, cholesterol, and liver triglyceride content than the CHOW. The water- and n-butanol extracts of OJ improved the elevated plasma triglyceride and glucose levels induced by high-fat highcholesterol diet and lowered triglyceride content in the liver. Similarly, the treatment with metadoxine reversed the increased plasma glucose and triglyceride and showed a tendency to decrease hepatic fat accumulation. However, these supplements did not change body weight or visceral fat mass. These results indicate that OJ extracts improve changes in hepatic fat accumulation, plasma glucose, and lipid profiles induced by high-fat high-cholesterol diet but did not affect visceral obesity. In conclusion, the water- and n-butanol extracts of OJ and metadoxine improved hepatic fat accumulation, hyperglycemia, and dyslipidemia induced by high-fat highcholesterol diet although the effect of metadoxine on fatty liver was not significant, suggesting their potential use for the prevention and treatment of NAFLD.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ALT:

alanine aminotransferase

AST:

aspartate aminotransferase

B:

n-butanol-extract

FABP:

fatty-acid binding protein

HFCD:

high-fat high-cholesterol diet group

M:

metadoxine

NAFLD:

nonalcoholic fatty liver disease model

OJ:

Oenanthe javanica

W:

water-extract

References

  • Angulo P (2002) Nonalcoholic fatty liver disease. N Engl J Med 346, 1221–1231.

    Article  CAS  Google Scholar 

  • Caballería J, Parés A, Brú C, Mercader J, García Plaza A, Caballería L, Clemente G, Rodrigo L, and Rodés J (1998) Metadoxine accelerates fatty liver recovery in alcoholic patients: results of a randomized double-blind, placebo-control trial. Spanish Group for the Study of Alcoholic Fatty Liver. J Hepatol 28, 54–60.

    Article  Google Scholar 

  • Carmiel-Haggai M, Cederbaum AI, and Nieto N (2003) Binge ethanol exposure increases liver injury in obese rats. Gastroenterology 125, 1818–1833.

    Article  CAS  Google Scholar 

  • Choudhury J and Sanyal AJ (2004) Insulin resistance and the pathogenesis of nonalcoholic fatty liver disease. Clin Liver Dis 8, 575–594.

    Article  Google Scholar 

  • Fehér J and Lengyel G (2003) A new approach to drug therapy in non-alcoholic steatohepatitis (NASH). J Int Med Res 31, 537–551.

    Google Scholar 

  • Fehér J, Váli L, Blázovics A, and Lengyel G (2009) The beneficial effect of metadoxine (pyridoxine-pyrrolidonecarboxylate) in the treatment of fatty liver diseases. Clin Exp Med J 3, 65–79.

    Article  Google Scholar 

  • Folch J, Sloane M, and Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497–509.

    CAS  Google Scholar 

  • Frayn KN and Maycock PF (1980) Skeletal muscle triacylglycerol in the rat: methods for sampling and measurement, and studies of biological variability. J Lipid Res 21, 139–144.

    CAS  Google Scholar 

  • Han YQ, Huang ZM, Yang XB, Liu HZ, and Wu GX (2008) In vivo and in vitro anti-hepatitis B virus activity of total phenolics from Oenanthe javanica. J Ethnopharmacol 118, 148–153.

    Article  CAS  Google Scholar 

  • Kadayifci A, Merriman RB, and Bass NM (2007). Medical treatment of non-alcoholic steatohepatitis. Clin Liver Dis 11, 119–140.

    Article  Google Scholar 

  • Kim JY, Kim KH, Lee YJ, Lee SH, Park JC, and Nam DH (2009) Accelerative ethanol Metabolism by Oenanthe javanica Extract in ethanol-treated animals BMB Rep 42, 482–485.

    Article  CAS  Google Scholar 

  • Koruk M, Savaf MC, Yilmaz O, Tayfi S, Karakok M, Gündoðdu C, and Yilmaz A (2003) Serum lipids, lipoproteins and apolipoproteins levels in patients with nonalcoholic steatohepatitis. J Clin Gastroenterol 37, 177–182.

    Article  CAS  Google Scholar 

  • Koteish A and Diehl AM (2001) Animal models of steatosis. Semin Liver Dis 21, 89–104.

    Article  CAS  Google Scholar 

  • Kral JG, Schaffner F, Pierson RN Jr, and Wang J (1993) Body fat topography as an independent predictor of fatty liver. Metabolism 42, 548–551.

    Article  CAS  Google Scholar 

  • Kwon DY, Jung YS, Kim SJ, Park HK, Park JH, and Kim YC (2009) Impaired sulfur-amino acid metabolism and oxidative stress in nonalcoholic fatty liver are alleviated by betaine supplementation in rats. J Nutr 139, 63–68.

    CAS  Google Scholar 

  • Lam BP and Younossi ZM (2009) Treatment regimens for non-alcoholic fatty liver disease. Ann Hepatol 8, S51-S59.

    Google Scholar 

  • Lazo M and Clark JM (2008) The epidemiology of nonalcoholic fatty liver disease: a global perspective. Semin Liver Dis 28, 339–350.

    Article  Google Scholar 

  • Lee L, Alloosh M, Saxena R, Van Alstine W, Watkins BA, Klaunig JE, Sturek M, and Chalasani N (2009) Nutritional model of steatohepatitis and metabolic syndrome in the Ossabaw miniature swine. Hepatology 50, 56–67.

    Article  CAS  Google Scholar 

  • Li SZ (1978) Compendium of Materia Medica. People’s Medical Publishing House, Beijing, China, pp. 1632–1633.

    Google Scholar 

  • Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, Uysal KT, Cao Q, Atsumi G, Malone H, Krishnan B, Minokoshi Y, Kahn BB, Parker RA, and Hotamisligil GS (2005) Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes. Cell Metab 1, 107–119.

    Article  CAS  Google Scholar 

  • Maeda K, Uysal KT, Makowski L, Görgün CZ, Atsumi G, Parker RA, Brüning J, Hertzel AV, Bernlohr DA, and Hotamisligil GS (2003) Role of the fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes 52, 300–307.

    Article  CAS  Google Scholar 

  • Malaguarnera M, Di Rosa M, Nicoletti F, and Malaguarnera L (2009) Molecular mechanisms involved in NAFLD progression. J Mol Med 87, 679–695.

    Article  CAS  Google Scholar 

  • Malhi H and Gores GJ (2008) Cellular and molecular mechanisms of liver injury. Gastroenterol 134, 1641–1654.

    Article  CAS  Google Scholar 

  • Marchi S, Polloni A, Costa F, Bellini M, Bonifazi V, Tumino E, Grassi B, Romano MR, De Bartolo G, Bertelli A, et al. (1990) Liver triglyceride accumulation after chronic ethanol administration: a possible protective role of metadoxina and ubiquinone. Int J Tissue React 12, 247–250.

    CAS  Google Scholar 

  • McCullough AJ (2002) Update on nonalcoholic fatty liver disease. J Clin Gastroenterol 34, 255–262.

    Article  Google Scholar 

  • Nishina PM, Lowe S, Wang J, and Paigen B (1994) Characterization of plasma lipids in genetically obese mice: the mutants obese, diabetes, fat, tubby, and lethal yellow. Metabolism 43, 549–553.

    Article  CAS  Google Scholar 

  • Park JC, Kim JY, Lee YJ, Lee JS, Kim BG, Lee SH, and Nam DH (2008) Protective effect Oenanthe javanica extract on acetaminophen-induced hepatotoxicity in rats. Yakhak Hoeji 52, 316–321.

    CAS  Google Scholar 

  • Park JC, Yu YB, Lee JH, Hattori M, Lee CK, and Choi JW (1996) Protective effect of Oenanthe javanica on the hepatic lipid peroxidation in bromobenzene-treated rats and its bioactive component. Planta Med 62, 488–490.

    Article  CAS  Google Scholar 

  • Polyzos SA, Kountouras J, and Zavos C. (2009) Nonalcoholic fatty liver disease: the pathogenetic roles of insulin resistance and adipocytokines. Curr Mol Med 9, 299–314.

    Article  CAS  Google Scholar 

  • Rector RS, Thyfault JP, Morris RT, Laye MJ, Borengasser SJ, Booth FW, and Ibdah JA (2008) Daily exercise increases hepatic fatty acid oxidation and prevents steatosis in Otsuka Long-Evans Tokushima Fatty rats. Am J Physiol Gastrointest Liver Physiol 294, G619–626.

    Article  CAS  Google Scholar 

  • Shoelson SE, Herrero L, and Naaz A (2007) Obesity, inflammation, and insulin resistance. Gastroenterol 132, 2169–2180.

    Article  CAS  Google Scholar 

  • Shulman GI (2000) Cellular mechanisms of insulin resistance. J Clin Invest 106, 171–176.

    Article  CAS  Google Scholar 

  • Torres DM and Harrison SA (2008) Diagnosis and therapy of nonalcoholic steatohepatitis. Gastroenterol 134, 1682–1698.

    Article  CAS  Google Scholar 

  • Uysal KT, Scheja L, Wiesbrock SM, Bonner-Weir S, and Hotamisligil GS (2000) Improved glucose and lipid metabolism in genetically obese mice lacking aP2. Endocrinol 141, 3388–3396.

    Article  CAS  Google Scholar 

  • Vuppalanchi R and Chalasani N (2009) Nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: Selected practical issues in their evaluation and management. Hepatology 49, 306–317.

    Article  Google Scholar 

  • Yang SQ, Lin HZ, Lane MD, Clemens M, and Diehl AM (1997) Obesity increases sensitivity to endotoxin liver injury: implications for the pathogenesis of steatohepatitis. Proc Natl Acad Sci USA 94, 2557–2562.

    Article  CAS  Google Scholar 

  • Yang XB, Huang ZM, Cao WB, Zheng M, Chen HY, and Zhang JZ (2000) Antidiabetic effect of Oenanthe javanica flavone. Acta Pharmacol Sin 21, 239–242.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Medicine, Yeungnam University, 705-717, Daegu, Republic of Korea

    Yun-Yeong Jeong, Youn Ju Lee, Keun-Mi Lee & Jong-Yeon Kim

Authors
  1. Yun-Yeong Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Youn Ju Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Keun-Mi Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jong-Yeon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Youn Ju Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jeong, YY., Lee, Y.J., Lee, KM. et al. The effects of Oenanthe javanica extracts on hepatic fat accumulation and plasma biochemical profiles in a nonalcoholic fatty liver disease model. J. Korean Soc. Appl. Biol. Chem. 52, 632–637 (2009). https://doi.org/10.3839/jksabc.2009.105

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.3839/jksabc.2009.105

Key words

Search

Navigation