Abstract
The occurrence of hyperhydricity in transgenic potato plants, which carry a chloroplastic Cu/ZnSOD gene of the lily in either a sense or antisense orientation, was investigated during in vitro culturing. Hyperhydric symptoms included curled leaves with a succulent appearance that were mainly observed in Cu/ZnSOD sense plants. By contrast, antisense plants showed a low frequency of hyperhydricity and a high rate of ex vitro survival. Therefore, a knockdown approach for a chCu/ZnSOD gene could be a practical method to reduce hyperhydricity in plant micropropagation.
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Abbreviations
- chCu/ZnSOD:
-
chloroplastic copper/zinc superoxide dismutase
- H2O2 :
-
hydrogen peroxide
- O2 ·- :
-
superoxide anion
- WT:
-
wild type
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Lee, YH., Kim, HS., Kim, MS. et al. Reduction of shoot hyperhydricity in micropropagated potato plants via antisense inhibition of a chCu/ZnSOD gene. J. Korean Soc. Appl. Biol. Chem. 52, 397–400 (2009). https://doi.org/10.3839/jksabc.2009.070
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DOI: https://doi.org/10.3839/jksabc.2009.070