Abstract
A calcium-binding peptide was purified from hydrolysates of porcine plasma protein. Porcine plasma protein was hydrolyzed using Flavourzyme, and the hydrolysates were filtered using YM-3 membrane. The membrane filtered solution was fractionated using Sephadex G-15, normal phase high-performance liquid, ion exchange chromatography, and fast protein liquid chromatography for a calcium-binding peptide. The sequence of the purified calcium-binding peptide was analyzed using liquid chromatography/electron spray ionization tandem mass spectrum and identified to be Val-Ser-Gly-Val-Glu-Asp-Val-Asn. The calcium- binding peptide can be used as a food ingredient or feed for calcium deficiency.
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Abbreviations
- BPP:
-
blood plasma protein
- CPPs:
-
casein phosphopeptides
- FPLC:
-
fast protein liquid chromatography
- HPLC:
-
high-performance liquid chromatography
- TNBS:
-
trinitrobenzene sulfornic acid
- PBPP:
-
porcine blood plasma protein
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Lee, S.H., Song, K.B. Article isolation of a calcium-binding peptide from enzymatic hydrolysates of porcine blood plasma protein. J. Korean Soc. Appl. Biol. Chem. 52, 290–294 (2009). https://doi.org/10.3839/jksabc.2009.051
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DOI: https://doi.org/10.3839/jksabc.2009.051