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Antioxidation effects of rosmarinic acid on human skin melanoma cells treated with hydrogen peroxide

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Abstract

The purpose of this study was to evaluate the antioxidant effect of rosmarinic acid on the cultured human skin melanoma cells injured by reactive oxygen species. The cytotoxicity and antioxidant effect were analyzed by XTT assay for cell adhesion activity, DPPH-radical scavenging activity, lactate dehydrogenase (LDH) activity after treated of cells with or without hydrogen peroxide (H2O2) for 1–10 h. H2O2 decreased the cell adhesion activity in cultured human melanoma cells in a dose- and time-dependent manner after cells were treated with 20–110 ώM of H2O2 for 5–7 h. IC50 values of H2O2 by XTT assay were 90 ώM, respectively. H2O2 increased LDH activity dose dependently. LDH50 was determined at 60 ώM H2O2 after treatment of cells with 50–90 ώM H2O2 for 8 h. Rosmarinic acid increases cell adhesion activity, DPPH-radical scavenging and decreases LDH activity. The treatment of the cells with H2O2 shows highly toxic effects on cultured human skin melanoma cells. Rosmarinic acid increases cell adhesion activity, DPPH-radical scavenging activity and decreases LDH activity in the cells treated with H2O2. It is considered that rosmarinic acid shows antioxidant effect on ROS such as H2O2.

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Abbreviations

LDH:

lactate dehydrogenase

MCV:

midpoint cytotoxicity value

RMA:

rosmarinic acid

RNS:

reactive nitrogen species

ROS:

reactive oxygen species

References

  • Andrade PB, Oliverira BM, Scabra RM, Ferreira MA, Ferreres F, and Garcia-viguera C (2001) Analysis of phenolic compounds in Spanish Albrarino and Portuguese Alvarinho and loureiro wines by capillary zone electrophoresis and high-performance liquid chromatofraphy. Electrophoresis 22, 1568–1572.

    Article  CAS  Google Scholar 

  • Borenfreund E, Babichi H, and Martin-Alcuacil N (1988) Comparisons of two in vitro cytotoxicity assay-the neutral red(NR) and tetrazolium MTT test. Toxicol In Vitro 2, 1–6.

    Article  CAS  Google Scholar 

  • Brune B, Zhou J, and Von Knethen (2003) A nitric oxide, oxidative stress, and apoptosis. Kidney Int Suppl 84, 22–24.

    Article  Google Scholar 

  • Chen S, Fu Y, and Wu R (1999) Effects of rosmarinic acid on free radical production and lysosomal enzyme release from rat peritoneal neutrophils. Acta Pharmacol Sin 34, 881–885.

    CAS  Google Scholar 

  • Delanty N and Dichter MA (1998) Oxidative injury in the nervous system. Acta Neurol Scand 98, 145–153.

    Article  CAS  Google Scholar 

  • Difazio MC, Hollingsworth Z, Young AB, and Penny JB (1992) Glutamate receptors in the substentia nigra of Parkinson’s disease brain. Neurology 42, 402–408.

    CAS  Google Scholar 

  • Duthie GG (1999) Parsley, polyphenols and nutritional antioxidants. Br J Nutr 81, 425–626.

    CAS  Google Scholar 

  • Heilmann J, Calis I, Kirmizibekmez H, Schuhly W, Harput S, and Sticher O (2000) Radical scavenger activity of phenylethanoid glycosides in FMLP stimulated human polymorphonuclear leukocytes: structure-activity relationships. Planta Med 66, 746–748.

    Article  CAS  Google Scholar 

  • Hirota A, Taki S, Kawaii S, Yano M, and Abe N (2000) 1-Diphenyl-2-picrylhydrazyl radical-scavenging compounds from soybean miso and antiproliferative acitvity of isoflavones from soybean miso toward the cancer cell lines. Biosci Biotechnol Biochem 64, 1038–1040.

    Article  CAS  Google Scholar 

  • Hruszkewycz AM (1998) Evidence for mitochondrial DNA damage by lipid peroxidation. Biochem Bioph Res Comm 153, 191–197.

    Article  Google Scholar 

  • Inoue M, Suzuki R, Sakaguchi N, Li Z, Takeda T, Ogihara Y, Jiang BY, and Chen Y (1995) Selective induction of cell death in cancer cells by gallic acid. Biol Pharm Bull 18, 1526–1530.

    Article  CAS  Google Scholar 

  • Kang YH, Park YK, Oh SR, and Moon KD (1995) Studies on the physiological functionality of pine needle and mugort extracts. Korean J Food Sci Technol 27, 978–984.

    Google Scholar 

  • Kim JB, Whang YS, Kim JB, Park ND, and Cho KJ (1999) Isolation, identification, and activity of rosmarinic acid, a potent antioxidant extracted from Korean Agastache rugosa. J Korean Agric Chem Soc 42, 262–266.

    CAS  Google Scholar 

  • Kim MS, Jin BK, Chun SW, Lee MY, Lee SH, Kim JH, and Park BR (1997) Role of verstibulo-cerebellar N-mehtyl-D-sapartate receptors for behavioral recovery following unilateral labyrinthectomy in rats. Neurosci Lett 222, 171–174.

    Article  CAS  Google Scholar 

  • Kim YS and Kim SU (1991) Oilgodendroglial cell death induced by oxygen radicals and its protection by catalase. J Neurosci Res 29, 100–106.

    Article  CAS  Google Scholar 

  • Mattson MP, Cheng B, and Sminth-Swintosky VL (1993) Mechanisms of neurotrophic factor protection against calcium-and free radical mediated excitotoxic injury: Implications for treating neurogenerative disorders. J Exp Neurol 124, 89–95.

    Article  CAS  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival Application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55–63.

    Article  CAS  Google Scholar 

  • Osakabe N, Yasuda A, Natsume M, Sanbongi C, Kato Y, Osawa T, and Yoshikawa T (2002) Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in d-galactosamine (d-GalN)-sensitized mice. Free Radical Bio Med 33, 798–806.

    Article  CAS  Google Scholar 

  • Pannala AS, Ricw-Evans CA, Halliwell B, and Singh S (1997) Inhibition of peroxvnitrite-mediatrd tyrosine nitration by catechin polyphenols. Biochem Bioph Res Comm 232, 164–170.

    Article  CAS  Google Scholar 

  • Park JI, Lee KS, Park MJ, Lee SH, and Kang SJ (1999) Isolation and quantitative determination of tanshinone IlA from the rhizomes of Salvia miltiorrhiza Bunge. Korean J Pharmacogn 30, 158–162.

    Google Scholar 

  • Saunders RD, Dugan LL, Demediuk P, Means ED, Harrocks LA, and Anderson DK (1987) Effects of methyl prednisolone and the combination of alpha-tocopherol and selenium on arachidonic acid metabolism and lipid peroxidation in traumatized spinal cord tissue. J Neurochem 49, 24–31.

    Article  CAS  Google Scholar 

  • Sandoval M, Zhang, XJ, Liu X, Mannick EE, Clark DA, and Miller MJ (1992) Peroxynitrite-induced apoptosis in T84 and RAW 264.7 cells: arrenuation by L-ascorbic acid. Free Radical Bio Med 22, 489–495.

    Article  Google Scholar 

  • Song WG, Kwon KB, Lee HS, Kim HC, Kim WK, Oh KS, An HC, and Ru DG (2001) Effects of Hwalrak-dan water extract on LDH activity of cultured spinal sensory neurons damaged by hydrogen peroxide. Korean J Orient Med Physiol Pathol 15, 464–468.

    Google Scholar 

  • Sun J, Chu YF, Wu X, and Liu RH (2002) Antioxidant and antiproliferative activities of commom fruits. J Agric Food Chem 50, 7449–7454.

    Article  CAS  Google Scholar 

  • Sung KC (2006) A study on the pharmacetical characteristics & analysis of Glycyrrhizin Extract. J Korean Oil Chemists Soc 23, 215–222.

    Google Scholar 

  • Yong HP (2006) Investigation of the antifibrotic effect of lithospermate B in hepatic stellate cells and liver cirrhosis animal model in rat. Korean J Hepatol 12, 80–84.

    Google Scholar 

  • Yokozawa T, Chung HY, Oura H, Nonaka G, and Nishioka I (1998) Effect of (dihydro) caffeic acid tetramer isolated from Salviae miltiorrhizae Radix on the renal function. J Med Pharm Soc wakan-yaku 5, 163–170.

    Google Scholar 

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Authors and Affiliations

  1. Department of Cosmetology, Dongkang College, 500-714, Gwangju, Republic of Korea

    Sun Mi Yoo

  2. Department of Bioengineering, Konkuk University, 143-701, Seoul, Republic of Korea

    Jeong Ran Kang

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  1. Sun Mi Yoo
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  2. Jeong Ran Kang
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Correspondence to Jeong Ran Kang.

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Yoo, S.M., Kang, J.R. Antioxidation effects of rosmarinic acid on human skin melanoma cells treated with hydrogen peroxide. J. Korean Soc. Appl. Biol. Chem. 52, 247–251 (2009). https://doi.org/10.3839/jksabc.2009.044

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  • DOI: https://doi.org/10.3839/jksabc.2009.044

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