Abstract
Origanum compactum Benth (Lamiaceae), is an endemic plant of northern Morocco. rich in phenolic compounds that is commonly used in traditional Moroccan medicine to treat several diseases. Several studies have shown that Origanum compactum has bioactive properties, including, antioxidant, cytotoxic, and antitumor activity. In this study, the aqueous infusion of Origanum compactum leaves was tested to evaluate the antiproliferative effect against the skin cancer cells line A431 (epidermoid carcinoma). The anti-inflammatory effect was also evaluated by the nitric oxide (NO) and antioxidant assay. For this, we determined the ability of Origanum extracts to inhibit the NO production by the microglial macrophage cells (BV2) stimulated by LPS (Lipopolysaccharide). The extracts showed a significant antiproliferative effect by reducing the cell viability percentage to 20%. In addition, the Origanum compactum infusion has potent antioxidant effects as tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, with an IC50 of 38.04 ± 0.52 µg/mL and reducing power of 252.96 ± 4.32 mg AAE/g DM (expressed as Acid Ascorbic Equivalents) evaluated by ferric-reducing antioxidant power (FRAP) assay. Also, the extracts induced a significant (p < 0.001) reduction in NO production by LPS-activated BV2 cells to 5.52 µM/mL compared to 17.12 µM/mL for non-activated control cells. The results obtained in this study show that Origanum compactum has anti-inflammatory and anti-tumorigenic properties that support its use in traditional medicine.
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Acknowledgements
The authors thank the Department of Immunology and Infection at Biomedical Research Institute, Hasselt University, Belgium.
Funding
This research project was supported by the Institutional University Cooperation Program at Moulay Ismail University (CUI- UMI) funded by the VLIR-UOS program at Hasselt University Belgium. The Project number was PAM-P6.
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Hamza, E.F., Mohamed, B., Hicham, M. et al. Antiproliferative, anti-inflammatory, and antioxidant activity of aqueous extracts from Origanum compactum benth aerial part. Vegetos (2023). https://doi.org/10.1007/s42535-023-00662-w
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DOI: https://doi.org/10.1007/s42535-023-00662-w