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Methylation status of CpG sites in the MCP-1 promoter is correlated to serum MCP-1 in Type 2 diabetes

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Abstract

Aim: Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine and plays an important role in atherosclerosis of Type 2 diabetes. The aim of this study was to investigate the methylation status of CpG sites in the MCP-1 promoter in Type 2 diabetic patients and its correlation to serum MCP-1 level, and blood glucose level. Methods: The 32 patients with Type 2 diabetes and 15 healthy controls were enrolled into the study. Body mass index, blood pressure, blood lipid, blood glucose, glycosylated hemoglobin (HbA1c), and serum MCP-1 were measured. Genomic DNA was isolated from the peripheral blood mononuclear cells (PBMC). Methylation status of CpG sites in the MCP-1 promoter was determined using methylation specific polymerase chain reaction. Results: The promoter region (2890–3050 bp) was predominantly methylated in PBMC from controls. Methylation of CpG motifs were less methylated in the patients than in the controls (25% vs 80%; p<0.001), while the level of MCP-1 in serum was higher in patients with Type 2 diabetes (193.95±74.96 vs 88.46±55.10; p<0.001). MCP-1 promoter methylation was significantly correlated to serum MCP-1, HbA1c, fasting blood glucose, and triglyceride. Conclusion: These data suggest that hypomethylation of CpG sites in the MCP-1 promoter region may be affected by blood glucose and TG, which then increase the serum MCP-1 level and may play a role in the vascular complications of Type 2 diabetes.

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Authors and Affiliations

  1. Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Avenue Jiefang 1277#, Wuhan 430022, Hubei, PR China

    Z. H. Liu, L. L. Chen, X. L. Deng, H. J. Song, Y. F. Liao, T. S. Zeng, J. Zheng & H. Q. Li MD

  2. Department of Endocrinology, The First Affiliated Hospital, Medical School, Yangtze University, Jingzhou, Hubei, PR China

    Z. H. Liu

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  1. Z. H. Liu
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  2. L. L. Chen
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  3. X. L. Deng
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  4. H. J. Song
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  5. Y. F. Liao
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Correspondence to H. Q. Li MD.

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Liu, Z.H., Chen, L.L., Deng, X.L. et al. Methylation status of CpG sites in the MCP-1 promoter is correlated to serum MCP-1 in Type 2 diabetes. J Endocrinol Invest 35, 585–589 (2012). https://doi.org/10.3275/7981

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  • DOI: https://doi.org/10.3275/7981

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