Abstract
The research was aimed at improving the technology of vegetative propagation of coniferous trees in vitro by selecting optimal media for sterilizing the explants of vegetative parts of plants and using growth regulators to obtain callus and stimulating the root formation of test-tube plants. The objects of study were the vegetative parts of conifers: the buds and parts of the annual shoots. Plant objects were sterilized and planted on nutrient media of different composition according to the scheme of experiments. Laboratory studies were conducted, and on their basis the successful use of sodium hypochlorite was noted compared with other reagents for sterilization of explants. According to the results of the experiments, a Woody Plant Medium nutrient medium was identified that promotes the best survival of the explants of conifers. The effect on growth survival of explants and their viability in culture in vitro, growth regulators such as 2,4-dichlorophenoxyacetic acid and 2,4-dinitrophenol in nutrient media was also determined. With further cultivation and reproduction of coniferous plants, this technique allowed for obtaining planting material that most economically preserved in the offspring the economically valuable traits and properties of the parent plant.
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Krasnoperova, V.V., Bukharina, I.L. The Study into the Method of Culture In Vitro as a Method of Vegetative Propagation of Coniferous Trees. Russ. Agricult. Sci. 46, 19–22 (2020). https://doi.org/10.3103/S1068367420010061
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DOI: https://doi.org/10.3103/S1068367420010061