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Investigating passive immunodiffusion reactions in the channels of a microfluidic device

  • Proceedings of the 19th National Conference on the Application of Synchrotron Radiation “SR-2012” and National Youth Conference “Application of Synchrotron Radiation”
  • Section of Terahertz Radiation
  • Published:
Bulletin of the Russian Academy of Sciences: Physics Aims and scope

Abstract

The passive immunodiffusion reaction in a microfluidic system is investigated via the interaction between fluorescein human albumin conjugate and monoclonal antibodies. The topology of a microfluidic device suitable for this reaction was designed, and the device was fabricated using LIGA technology. A system for transitioning from the macro- to the microlevel is developed and produced. The dissociation constants and specificity of interaction in complexes formed as a result of the reaction between fluorescein human albumin conjugates and monoclonal antibodies are determined. The results from our study reveal that systems based on combinations of passive immunodiffusion reactions and microfluidic technologies are applicable for detecting antigens and antibodies in the analyzed fluids.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    S. E. Peltek, T. N. Goryachkovskaya, S. V. Bannikova & S. V. Shekhovtsov

  2. Budker Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    B. G. Goldenberg & V. M. Popik

  3. Technological Design Institute of Scientific Instrument Engineering, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630058, Russia

    A. K. Potashnikov

Authors
  1. S. E. Peltek
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  2. T. N. Goryachkovskaya
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  3. S. V. Bannikova
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  4. S. V. Shekhovtsov
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  5. B. G. Goldenberg
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  6. A. K. Potashnikov
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  7. V. M. Popik
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Corresponding author

Correspondence to V. M. Popik.

Additional information

Original Russian Text © S.E. Peltek, T.N. Goryachkovskaya, S.V. Bannikova, S.V. Shekhovtsov, B.G. Goldenberg, A.K. Potashnikov, V.M. Popik, 2013, published in Izvestiya Rossiiskoi Akademii Nauk. Seriya Fizicheskaya, 2013, Vol. 77, No. 9, pp. 1341–1344.

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Peltek, S.E., Goryachkovskaya, T.N., Bannikova, S.V. et al. Investigating passive immunodiffusion reactions in the channels of a microfluidic device. Bull. Russ. Acad. Sci. Phys. 77, 1171–1174 (2013). https://doi.org/10.3103/S1062873813090360

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  • DOI: https://doi.org/10.3103/S1062873813090360

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