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Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress

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Abstract

The expression of the OmpF porin gene in Yersinia pseudotuberculosis in response to antibiotics of two different classes (kanamycin and nalidixic acid) was analyzed using a quantitative PCR and a fluorescence reporter system. Both antibiotics downregulated the expression of the ompF gene. The nalidixic acid significantly reduced the ompF expression, while kanamycin, for which porins are considered to be an alternative transport route, only slightly reduced the ompF level.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Vladivostok, 690022, Russia

    E. P. Bystritskaya, A. M. Stenkova, O. Yu. Portnyagina, V. A. Rasskazov & M. P. Isaeva

  2. Far Eastern Federal University, ul. Sukhanova 8, Vladivostok, 690950, Russia

    A. M. Stenkova, O. Yu. Portnyagina & M. P. Isaeva

  3. Max von Pettenkofer Institute for Hygiene and Clinical Microbiology, Ludwig Maximilian University, Pettenkoferstrasse 9a, Munich, D-80336, Germany

    A. V. Rakin

Authors
  1. E. P. Bystritskaya
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  2. A. M. Stenkova
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  3. O. Yu. Portnyagina
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  4. A. V. Rakin
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  5. V. A. Rasskazov
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  6. M. P. Isaeva
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Correspondence to A. M. Stenkova.

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Original Russian Text © E.P. Bystritskaya, A.M. Stenkova, O.Yu. Portnyagina, A.V. Rakin, V.A. Rasskazov, M.P. Isaeva, 2014, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2014, No. 2, pp. 17–21.

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Bystritskaya, E.P., Stenkova, A.M., Portnyagina, O.Y. et al. Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress. Mol. Genet. Microbiol. Virol. 29, 63–68 (2014). https://doi.org/10.3103/S0891416814020037

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  • DOI: https://doi.org/10.3103/S0891416814020037

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