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Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes

  • Microbiology
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Abstract

It has been shown that micromycetes Aspergillus ustus 1 and Tolypocladium inflatum k1 secrete proteolytic enzymes that possess high collagenolytic, fibrinolytic, and elastolytic activity. The activity of proteinases hydrolyzing fibrillar proteins, which was determined by the cleavage of azo-collagen, was 122.6 × 10–3EAzc/mL in A. ustus 1 and 69.7 × 10–3EAzc/mL in T. inflatum k1 (EAzc is the amount of azocollagen cleaved in 1 min (μg). The maximum values of activity were observed during submerged cultivation of A. ustus 1 for 4 days and of T. inflatum k1 for 5 days. It has been shown that the maximum of collagenolytic and general proteolytic activity during the cultivation of A. ustus 1 are time-separated, unlike T. inflatum k1, which, presumably, can simplify the procedure for obtaining proteinases active against fibrillar proteins.

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Authors and Affiliations

  1. Department of Biology, Moscow State University, Moscow, 119234, Russia

    E. A. Popova, D. M. Bednenko, A. A. Osmolovskiy, V. G. Kreyer & I. B. Kotova

  2. International Biotechnology Center, Moscow State University, Moscow, 119234, Russia

    N. S. Egorov

Authors
  1. E. A. Popova
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  2. D. M. Bednenko
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  3. A. A. Osmolovskiy
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  4. V. G. Kreyer
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  5. I. B. Kotova
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  6. N. S. Egorov
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Corresponding author

Correspondence to A. A. Osmolovskiy.

Additional information

Original Russian Text © E.A. Popova, D.M. Bednenko, A.A. Osmolovskiy, V.G. Kreyer, I.B. Kotova, N.S. Egorov, 2017, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2017, Vol. 72, No. 4, pp. 241–245.

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Popova, E.A., Bednenko, D.M., Osmolovskiy, A.A. et al. Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes. Moscow Univ. Biol.Sci. Bull. 72, 206–210 (2017). https://doi.org/10.3103/S0096392517040101

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  • DOI: https://doi.org/10.3103/S0096392517040101

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