Abstract
The polymorphism of beta-amylase isoenzymes in spring common wheat was studied among varieties cultivated under the conditions of the Northern Trans-Urals. Four zymotypes were identified among the 41 accessions. A, C, and A* isozymes differed most frequently, the share of which, respectively, was 64.6 ± 7.3, 22.0 ± 3.7, and 12.2 ± 3.7%. During 2011–2013, the aggregating ability of grain proteins was studied using -S-S-bonds between groups of varieties carrying variants of beta-amylase A and C. It was established that the presence of isoenzyme C promoted a greater aggregation of grain polypeptides in comparison with beta-amylase A carriers but did not affect the formation of yield. Differences in beta-amylase did not affect the falling number associated with amylase activity.
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REFERENCES
Sopanen, T. and Lauriere, C., Release and activity of bound β-amylase in a germinating barley grain, Plant Physiol., 1989, vol. 89, pp. 244–249. doi 0032-0889/ 89/89/0244/06/$01.00/0
Joudrier, P.M. and Bernard, M., Responsability du genome D sur certaines isozymes β-amylase du grain de ble tender, Ann. Amelior. Plantes, 1977, vol. 27, p. 35.
Rybalka, A.I., The Quality of Wheat and Its Improvement, Kiev: Logos, 2011.
Gupta, R.B., Shepherd, K.W., and MacRitchie, F., Genetic control and biochemical properties of some high molecular weight albumins in bread wheat, J. Cer. Sci., 1991, vol. 13, no. is. 3, pp. 221–235.https://doi.org/10.1016/S0733-5210(09)80002-7
Netsvetaev, V.P., Akinshina, O.V., Bondarenko, L.S., and Motorina, I.P., The β-amylase polymorphism of winter common wheat grains, Russ. J. Genet., 2012, vol. 48, no. 2, pp. 146–151. https://doi.org/10.1134/S10227-9541202010X
Netsvetaev, V.P., Akinshina, O.V., and Bondarenko, L.S., Use of the common winter wheat homozygous population for genetic analysis of beta-amylase and evaluation of its aggregation ability, Russ. J. Genet., 2014, vol. 50, no. 11, pp. 1156–1160. https://doi.org/10.1134/S1022795414110106
Netsvetaev, V.P., Nerubenko, O.E., Bondarenko, L.S., Akinshina, O.V., et al., Heterogeneity of the wheat variety as the basis for its improvement in the primary seed production, Achievements Sci. Technol. Agro-Industrial Complex, 2017, vol. 31, no. 6, pp. 43–46.
Netsvetaev, V.P., Lutenko, O.V., Pashchenko, L.S., and Popkova, I.I., Sedimentation methods and evaluation of the quality in common wheat gluten, Sci. Bull. Bel. Gos. Univ., Ser.: Nat. Sci. (Belgorod), 2009, vol. 11 (66), no. 9 (1), pp. 56–64.
Poperelya, F.A., Asyka, Yu.A., and Lazarev, Yu.D., The device for electrophoresis of proteins in the gel, Author. Witness USSR, 1991, no. 1 682 899.
Netsvetaev, V.P., Position of the β-amylase locus, Bmy 1, on barley chromosome 4, Cytol. Genet., 1993, vol. 27, no. 5, pp.74–78.
Rokitsky, P.F., Biological Statistics, Minsk: Vysheyshaya Shkola, 1973.
Rokitsky, P.F., Introduction to Statistical Genetics, Minsk: Vysheyshaya Shkola, 1974.
Li, Ch.Ch., First Course in Population Genetics, Pacific Grove, California: The Boxwood Press, 1976.
Netsvetaev, V.P., Motorina, I.P., and Petrenko, A.V., Comparison of methods for determining the quality of grains gluten in common wheat by the device IDK-1, Rep. RAAS, 2005, no. 4, pp. 14–16.
Netsvetaev, V.P., Motorina, I.P., and Petrenko, A.V., Modification of the method for determining the quality of wheat gluten on an IDK-1 device, Sci. Bull. Bel. Gos. Univ., Ser.: Nat. Sci. (Belgorod), 2006, no. 23/4 (3), pp. 141–144.
Netsvetaev, V.P., Ryzhkova, T.A., and Tretyakov, M.Yu., Quality of Common Wheat: Genetics and Breeding. Monograph, Belgorod: Otchiy Kray, 2015.
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Akhtariyeva, M.K., Kozelets, Y.O., Filippova, Y.M. et al. Beta-Amylase Isozymes in Spring Common Wheat and Their Role in the Aggregation of Grain Proteins. Cytol. Genet. 53, 294–299 (2019). https://doi.org/10.3103/S0095452719040029
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DOI: https://doi.org/10.3103/S0095452719040029