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Use of the common winter wheat homozygous population for genetic analysis of beta-amylase and evaluation of its aggregation ability

  • Plant Genetics
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Abstract

We investigated a self-pollinated homozygous population of common winter wheat, F→∞ 24/04 × Odesskaya krasnokolosaya, for variants of beta-amylase and the aggregation ability of the protein complex of seed via disulfide bonds. It was found that variation in the electrophoretic types of this enzyme was due to four isoenzymes. Two of them (a and b) are doubled and controlled by separate loci with independent inheritance. Isoenzyme c was due to three dominant factors, and four loci were responsible for d. Analysis of the number of -S-S- bonds of five genotypes, which were harvested in 2013 and differed in the types of beta-amylase, showed that some of them were significantly different from others in this indicator. In general, the samples were grouped by the type of this enzyme, forming the following continuous series with respect to aggregation ability: I ≥ B ≥ F ≥ D ≥ G or 59.13 ± 3.18 ≥ 56.65 ± 2.46 ≥ 52.54 ± 2.24 ≥ 50.16 ± 1.67 ≥ 48.63 ± 6.25 of cond. units. Significant differences were observed for this property between groups B > D and I > D. Therefore, genotypes having types I and B have a positive influence on the rheological properties of dough.

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References

  1. Ainsworth, C.C., Gale, M.D., and Baird, S., The genetics of beta-amylase isozymes in wheat: allelic variation among hexaploid varieties and intrachromosomal gene locations, Theor. Appl. Genet., 1983, vol. 66, pp. 39–49.

    Article  CAS  PubMed  Google Scholar 

  2. Sharp, P.J., Desai, S., and Gale, M.D., Isozyme variation and RFLPs at the beta-amylase loci in wheat, Theor. Appl. Genet., 1988, vol. 76, pp. 691–699.

    Article  CAS  PubMed  Google Scholar 

  3. McIntosh, R.A., Hart, G.E., Devos, K.M., et al., Catalogue of gene symbols for wheat, in Proceedings of the 9th International Wheat Genetics Symposium, Saskatchewan, 1998, vol. 5, pp. 88, 155–156.

    Google Scholar 

  4. Rybalka, A.I. and Sozinov, A.A., Genetic analysis of beta-amylase in wheat grain, Genetika (Moscow), 1980, vol. 16, no. 6, pp. 1059–1067.

    CAS  Google Scholar 

  5. Rybalka, O.I., Yakist’ pshenitsi ta ii polipshennya (Wheat Quality and Its Improvement), Kiiv: Logos, 2011, pp. 234–240.

    Google Scholar 

  6. Gupta, R.B., Shepherd, K.W., and Macritchie, F., Genetic control and biochemical properties of some high molecular weight albumins in bread wheat, J. Cereal Sci., 1991, vol. 13, no. 3, pp. 221–235.

    Article  CAS  Google Scholar 

  7. Gianibelli, M.C. and Larroque, O.R., Biochemical, genetic, and molecular characterization of wheat endosperm proteins, Publication no. C-2001-092601O. http://www.aaccnet.org/cerealchemistry/freeart-icle/gianibelli.pdf

  8. Netsvetaev, V.P., Akinshina, O.V., Bondarenko, L.S., et al., The β-amylase polymorphism of winter common wheat grains, Russ. J. Genet., 2012, vol. 48, no. 2, pp. 146–151.

    Article  CAS  Google Scholar 

  9. Ryzhkova, T.A., Bondarenko, L.S., Akinshina, O.V., et al., The role of disulphide bond number of protein complex in the control of wheat grain quality, in Impactul Realizǎrilor Ştiintifice Asupra Producţiei şi Calitǎţii Cerealelor Spicoase, Bǎlţi, 2013, pp. 177–182.

    Google Scholar 

  10. Netsvetaev, V.P., Lyutenko, O.V., Pashchenko, L.S., and Popkova, I.I., Sedimentation methods and the assessment of common wheat gluten quality, Nauchnye vedomosti belgorodskgo gosudarstvennogo universiteta, seriya estestvennye nauki (Scientific Gazette of Belgorod State University, Series Natural Sciences), Belgorod: Belgorod Gos. Univ., 2009, no. 11 (66), issue 9/1, pp. 56–64.

    Google Scholar 

  11. Netsvetaev, V.P., Lyutenko, O.V., Pashchenko, L.S., and Popkova, I.I., Estimation of grain quality in common wheat variants by SDS-sedimentation, S-kh. Biol., 2010, no. 3, pp. 63–70.

    Google Scholar 

  12. Rokitskii, P.F., Biologicheskaya statistika (Biological Statistics), Minsk: Vysheishaya shkola, 1973.

    Google Scholar 

  13. Rokitskii, P.F., Vvedenie v statisticheskuyu genetiku (Introduction to Statistical Genetics), Minsk: Vysheishaya shkola, 1974.

    Google Scholar 

  14. Persson, G., An attempt to find suitable genetic markers for dense ear loci in barley I, Hereditas, 1969, vol. 62, no. 3, pp. 25–96.

    Google Scholar 

  15. Netsvetaev, V.P., Rukovodstvo po geneticheskomu analizu rastenii (Manual on Plant Genetic Analysis), Belgorod: Belgorod Gos. Univ., 2008.

    Google Scholar 

  16. Netsvetaev, V.P., Lyutenko, O.V., Pashchenko, L.S., and Popkova, I.I., New approaches to assessment of common winter wheat grain quality, Belgorodskii Agromir (Belgorod Agricultural World), 2010, no. 4 (56), pp. 27–29.

    Google Scholar 

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Authors and Affiliations

  1. Belgorod State Research Institute of Agriculture, Russian Academy of Agricultural Sciences, Belgorod, 308001, Russia

    V. P. Netsvetaev, O. V. Akinshina & L. S. Bondarenko

  2. Department of Medicine and Biology, Belgorod State University, 308015, Belgorod, Russia

    V. P. Netsvetaev, O. V. Akinshina & L. S. Bondarenko

Authors
  1. V. P. Netsvetaev
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  2. O. V. Akinshina
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  3. L. S. Bondarenko
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Correspondence to V. P. Netsvetaev.

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Original Russian Text © V.P. Netsvetaev, O.V. Akinshina, L.S. Bondarenko, 2014, published in Genetika, 2014, Vol. 50, No. 11, pp. 1305–1309.

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Netsvetaev, V.P., Akinshina, O.V. & Bondarenko, L.S. Use of the common winter wheat homozygous population for genetic analysis of beta-amylase and evaluation of its aggregation ability. Russ J Genet 50, 1156–1160 (2014). https://doi.org/10.1134/S1022795414110106

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  • DOI: https://doi.org/10.1134/S1022795414110106

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