Abstract
The genetic variability of an amaranth collection was studied by RAPD analysis. A high level of polymorphism of the studied samples was detected. It was about 85% on average. Unique fragments characteristic of only definite accessions and 18 monomorphic loci proper for all amaranth accessions were detected using several primers. The genetic distances of Nei and Li were calculated. This index varied from 0.0009 to 0.0141. A cluster analysis was carried out. The amaranth accessions were classified into two clusters according to the species affiliation.
Similar content being viewed by others
References
Goptsii, T.I., Amaranth: Biology, Cultivation, Prospects of Use, and Breeding, Kharkiv, 1999.
Altukhov, Yu.P. and Salmenkova, E.A., DNA Polymorphism in Population Genetics, Russ. J. Genet., 2002, vol. 38, no. 9, pp. 1173–1195.
Kalendar’, R.N. and Glazko, V.I., Types of Molecular-Genetic Markers and Their Application, Fiziol. Biokhim. Kul’tur. Rast., 2002, vol. 34, no. 4, pp. 279–295.
Malyshev, S.V. and Kartel’, N.A., Molecular Markers in Genetic Mapping of Plants, Mol. Biol. (Moscow), 1997, vol. 31, no. 2, pp. 197–208.
Kochieva, E.Z., The Use of PCR-Based Methods for Analysis and Mapping of Plant Genome, S.-Kh. Biol., 1999, no. 3, pp. 3–14.
Ray, T. and Roy, S.C., Genetic Diversity of Amaranthus Species from the Indo-Gengetik Plains Revealed by RAPD Analysis Leading to the Development of Ecotype-Specific SCAR Marker, J. Hered., 2009, vol. 100, no. 3, pp. 338–347.
Chan, K.F. and Sun, M., Genetic Diversity and Relationships Detected by Isozyme and RAPD Analysis of Crop and Wild Species of Amaranthus, Theor. Appl. Genet., 1997, vol. 95, nos. 5/6, pp. 865–873.
Mandal, N. and Das, P.K., Intra- and Interspecific Genetic Diversity in Grain Amaranthus Using Random Amplified Polymorphic DNA Markers, Plant Tissue Culture, 2002, vol. 12, no. 1, pp. 49–56.
Ranade, S.A., Kumar, A., Goswamt, M., Farooqui, N., and Sane, P.V., Genome Analysis of Amaranth: Determination of Inter- and Intra-Species Variation, J. Biosci., 1997, vol. 22, no. 4, pp. 457–464.
Stefunova, I.V., Genetic Analysis of Amaranth (Amaranthus L.) using DNA Markers, Extended Abstract of Dissertation in Genetics, Nitra, 2008
Faseela, K.V. and Salikutty, J., Molecular Characterization of Amaranthus landraces and Assessment of Interspecific Relationships among Amaranthus spp. L. Using RAPD Markers, Indian J. Genet., 2007, vol. 67, no. 1, pp. 12–17.
Mallory, M.A., Hall, R.V., McNabb, A.R., Pratt, D.B., Jellen, E.N., and Maughan, P.J., Development and Characterization of Microsatellite Markers for the Grain Amaranths, Crop Sci., 2008, vol. 48, no. 3, pp. 1098–1106.
Xu, F. and Sun, M., Comparative Analysis of Phylogenetic Relationships of Grain Amaranths and Their Wild Relatives (Amaranthus; Amaranthaceae) Using Internal Transcribed Spacer, Amplified Fragment Length Polymorphism, and Double-Primer Fluorescent Intersimple Sequence Repeat Markers, Mol. Phylogenet. Evol., 2001, vol. 21, no. 3, pp. 372–387.
Ispol’zovanie PTsR-analiza v genetiko-selektsionnykh issledovaniyakh: Nauch.-metod. rukovodstvo (The Use of PCR Analysis in Genetic and Breeding Studies: Scientific and Methodical Guidelines), Sivolap, Yu.M., Ed., Kyiv: Agrar. Nauka, 1998.
Nei, M. and Li, W.-H., Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases, Proc. Nat. Acad. Sci. U.S.A., 1979, vol. 76, no. 10, pp. 5269–5273.
Goptsiy, T., Voroncov, N., Popov, V., Zhyravel, D., and Gromenko, S., Grain Varieties of Amaranth Developed by Selection at Kharkiv National Agrarian University and the Perspectives of Their Use, in Amaranth-Plant of the Future: 5th Int. Symp. of the European Amaranth Association, Nitra, 2008, pp. 97–100.
Sauer, J.D., The Grain Amaranths and Its Relatives: A Revised Taxonomic and Geographic Survey, Ann. Missour. Bot. Gard., 1967, vol. 54, pp. 102–137.
Sauer, J.D., Grain Amaranth, in Evolution of Crop Plants, Simmonds, N.W., Ed., London: Longman Group Ltd., 1976, pp. 4–7.
Greizerstein, E.I. and Poggio, L., Karyological Studies in Grain Amaranths, Cytologia, 1994, vol. 59, no. 1, pp. 25–30.
Kulakow, P.A., Hauptli, H., and Jain, S.K., Genetics of Grain Amaranths. I. Mendelian Analysis of Six Color Characteristics, J. Hered., 1985, vol. 76, no. 1, pp. 27–30.
Hauptli, H. and Jain, S.B., Genetic Structure of Landrace Populations of the New World Grain Amaranths, Euphytica, 1984, vol. 33, no. 3, pp. 875–884.
Lanoue, K.Z., Wolf, P.G., Browning, S., and Hood, E.E., Phylogenetic Analysis of Restriction-Site Variation in Wild and Cultivated Amaranthus Species (Amaranthaceae), Theor. Appl. Genet., 1996, vol. 93, pp. 722–732.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S.V. Lymanska, 2012, published in Tsitologiya i Genetika, 2012, Vol. 46, No. 4, pp. 19–26.
About this article
Cite this article
Lymanskaya, S.V. Estimation of the genetic variability of an amaranth collection (Amaranthus L.) by RAPD analysis. Cytol. Genet. 46, 210–216 (2012). https://doi.org/10.3103/S0095452712040093
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0095452712040093