Abstract
The gene sequence encoding the mannose-binding homodimeric protein Allium sativum leaf agglutinin (ASAL) was introduced into maize inbred lines to achieve resistance against sap-sucking corn leaf aphids in transgenic lines. Maize transformants were generated after the co-cultivation of immature maize embryos with the Agrobacterium strain LB4404 harbouring a recombinant Ti-binary ASAL construct driven by the polyubiquitin promoter. The preliminary screening of maize transformants was performed via GUS histochemical analysis, and PCR and Southern blotting confirmed the insertion of the transgene and its stable integration in five transgenic maize lines. Transcript abundance was quantified by a quantitative real-time PCR assay, which revealed variable expression of the ASAL transgene among five transgenic maize lines. The highest mRNA expression of the ASAL gene was found in the A23 transgenic maize line, while the lowest expression was found in the AU1 transgenic maize line. In planta bioassays in the T1 progeny of the transgenic maize lines revealed high resistance against corn leaf aphids compared to the control non-transgenic line. The mortality of the infesting aphids (Rhopalosiphum maidi) was found to vary from 40 to 71% compared to that of the non-transgenic control maize line.
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Abbreviations
- ASAL:
-
Allium sativum leaf agglutinin
- GUS:
-
β-glucuronidase
- PCR:
-
Polymerase chain reaction
- TMVΩ:
-
TMVΩ translational enhancer
- T1 :
-
first generation progeny plants
- Bt:
-
Bacillus thuringenesis
- NOS:
-
nopaline synthase gene
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Bhatti, M.U., Riaz, S., Toufiq, N. et al. The potential and efficacy of Allium sativum leaf lectin (ASAL) against sap-sucking insect pests of transgenic maize. Biologia 75, 2351–2358 (2020). https://doi.org/10.2478/s11756-020-00533-8
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DOI: https://doi.org/10.2478/s11756-020-00533-8